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Probe A shown in the GeneXpert MTB/RIF assay during the detection of Mycobacterium intracellular infections
- Source :
- Diagnostic Microbiology and Infectious Disease. 99:115243
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Mycobacterium tuberculosis (MTB) is commonly diagnosed via the GeneXpert MTB/RIF assay. The cycle threshold (Ct) value of probe A from this assay produced a fluorescence signal upon Mycobacterium intracellulare detection. No other nontuberculous mycobacteria (NTM) exhibited positive probe signals. Using a confirmed mycobacterial culture as a standard, probe A of the assay exhibited 84% sensitivity (95% confidence interval [CI]: 71%–97%) and 50% specificity (95% CI: 37%–63%) for clinical samples. For M. intracellulare strains, probe A exhibited 90% sensitivity (95% CI: 80%–100%) and 50% specificity (95% CI: 37%–63%). The identity of the amino acid sequence and 81-bp core region of rpoB from MTB and NTM suggested that the highly conserved property might be associated with a mismatch between the probes and the chromosomal DNA target. Probe A yielded a positive signal upon M. intracellulare detection; thus, probe A may help diagnose M. intracellular infections.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
030106 microbiology
Sensitivity and Specificity
Mycobacterium tuberculosis
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Drug Resistance, Bacterial
Humans
030212 general & internal medicine
Mycobacterium avium-intracellulare Infection
Bacteriological Techniques
GeneXpert MTB/RIF
biology
Chemistry
Mycobacterial culture
Nontuberculous Mycobacteria
General Medicine
Mycobacterium avium Complex
bacterial infections and mycoses
rpoB
biology.organism_classification
Molecular biology
Infectious Diseases
Molecular Diagnostic Techniques
Chromosomal dna
Nontuberculous mycobacteria
Rifampin
Sequence Analysis
Intracellular
Mycobacterium
Subjects
Details
- ISSN :
- 07328893
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- Diagnostic Microbiology and Infectious Disease
- Accession number :
- edsair.doi.dedup.....546e8b0a0c9bc8de8aec15027419dccb