Back to Search
Start Over
Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response
- Source :
- Veterinary Immunology and Immunopathology. 237:110269
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties in humans and animals. In broilers, CP supplementation improves energy utilization and nutrient digestibility, but limited information on its effects on chicken immunity is available so far. This study aimed to assess the in vitro impact of CP on chicken monocytes' immune response. Cells were purified from whole blood of healthy chickens and incubated with increasing concentrations (0, 0.25, 0.5, 0.75, 1 mg/mL) of CP to determine CP working concentration. The effects of different CP concentrations on cells' apoptosis and viability were assessed by measuring caspase-3 and -7 and the cells' metabolic activity (MTT assay), respectively. CP had no dose-dependent effect on monocyte apoptosis and viability.Then, the effects of CP (0.5 mg/mL) on chicken monocytes' chemotaxis and phagocytosis were assessed by measuring transwell migration and fluorescein-labelled E. coli incorporation, respectively. CP inhibited both monocytes' chemotaxis and phagocytosis.These data demonstrate that CP exerts an immunomodulatory role in chicken monocytes, supporting its integration in nutrition strategies that might be beneficial for the animal's immunity and health.
- Subjects :
- Citrus
food.ingredient
Pectin
Cell Survival
040301 veterinary sciences
Phagocytosis
Immunology
Apoptosis
Biology
Monocytes
Microbiology
0403 veterinary science
03 medical and health sciences
food
Immune system
medicine
Animals
Immunologic Factors
MTT assay
Citrus Pectin
Cells, Cultured
030304 developmental biology
0303 health sciences
General Veterinary
Monocyte
Anti-Inflammatory Agents, Non-Steroidal
Chemotaxis
04 agricultural and veterinary sciences
Chemotaxis, Leukocyte
medicine.anatomical_structure
Pectins
Chickens
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 237
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....546354b31867f477ce78ddf2d833dddc