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Effect of belt electrode-skeletal muscle electrical stimulation on immobilization-induced muscle fibrosis
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 5, p e0244120 (2021)
- Publication Year :
- 2020
-
Abstract
- Purpose: Macrophage accumulation in response to decreasing myonuclei may be the major mechanism underlying immobilization-induced muscle fibrosis in muscle contracture, an intervention strategy suppressing these lesions is necessary. Therefore, this research investigated the effect of belt electrode-skeletal muscle electrical stimulation (B-SES), a new electrical stimulation device, to the macrophage accumulation via myonuclei decrease in immobilization-induced muscle fibrosis. Materials and methods: 18 Wistar male rats were divided into the control group, immobilization group (with plaster cast fixation to immobilize the soleus muscles in a shortened position for 2 weeks), and B-SES group (with muscle contractile exercise through B-SES during the immobilization period). B-SES stimulation was performed at a frequency of 50 Hz and an intensity of 4.7 mA, muscle contractile exercise by B-SES was applied to the lower limb muscles for 20 minutes/session (twice a day) for 2 weeks (6 times/week). The bilateral soleus muscles were used for histological, immunohistochemical, biochemical, and molecular biological analyses. Results: The number of myonuclei was significantly higher in the B-SES group than in the immobilization group, and there was no significant difference between the B-SES and control groups. The cross-sectional area of type I and II myofibers in the immobilization and B-SES groups was significantly lower than that in the control group, and the cross-sectional area of type I myofibers in the B-SES group was higher than that in the immobilization group. However, Atrogin-1 and MuRF-1 mRNA expression in the immobilization and B-SES groups was significantly higher than those in the control group. Additionally, the number of macrophages, IL-1β, TGF-β1, and α-SMA mRNA expression, and hydroxyproline expression was significantly lower in the control and B-SES groups than those in the immobilization group. Conclusion: This research surmised that muscle contractile exercise through B-SES prevented immobilization-induced muscle fibrosis, and this alteration suppressed the development of muscle contracture.<br />PLoS ONE, 16(5), art. no. e0244120; 2021
- Subjects :
- 0301 basic medicine
Male
Muscle Physiology
Physiology
Interleukin-1beta
Muscle Fibers, Skeletal
Stimulation
chemistry.chemical_compound
White Blood Cells
0302 clinical medicine
Fibrosis
Animal Cells
Medicine and Health Sciences
Morphogenesis
Range of Motion, Articular
Musculoskeletal System
Chemokine CCL2
Fixation (histology)
Multidisciplinary
Muscles
Muscle Biochemistry
Muscle Differentiation
Hydroxyproline
medicine.anatomical_structure
Immunohistochemistry
Medicine
medicine.symptom
Anatomy
Cellular Types
Muscle fibrosis
Muscle contraction
Research Article
Muscle Contraction
medicine.medical_specialty
Science
Immune Cells
Immunology
Surgical and Invasive Medical Procedures
Transforming Growth Factor beta1
03 medical and health sciences
Immobilization
Internal medicine
medicine
Functional electrical stimulation
Animals
RNA, Messenger
Rats, Wistar
Muscle, Skeletal
Electrodes
Muscle contracture
Blood Cells
Functional Electrical Stimulation
business.industry
Macrophages
Significant difference
Skeletal muscle
Biology and Life Sciences
Soleus Muscles
Cell Biology
medicine.disease
Actins
Electric Stimulation
030104 developmental biology
Endocrinology
chemistry
Skeletal Muscles
Joints
Ankle
business
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....5442fb12af34fd6b92cf715f405d833c