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Diagnostic and Prognostic Potential of Serum Cell-Free microRNA-214 in Glioma
- Source :
- World neurosurgery. 125
- Publication Year :
- 2018
-
Abstract
- Objective We aimed to expound feasibility of serum cell-free microRNA-214 (miR-214) as a noninvasive biomarker for glioma in this study. Patients and Methods We detected expression of miR-214 in medium from 2 glioma cell lines to confirm whether it is secretory in screening phase. Then, we verified cell-free miR-214 expression in serum samples from an independent set of 100 preoperative patients with glioma, 30 matching postoperative patients, and 100 healthy controls. Results MiR-214 was secreted from glioma cell lines. Extracellular miR-214 levels were significantly overexpressed in preoperative serum from glioma patients with glioma, whereas its expression significantly decreased in matched postoperative serum. Upregulated cell-free miR-214 in serum was significantly associated with higher tumor grade, absence of isocitrate dehydrogenase, and unmethylated methylguanine methyltransferase promoter. Extracellular miR-214 in serum could effectively distinguish patients with glioma from healthy control (area under the curve = 0.885; 95% confidence interval, 0.833–0.926). Moreover, serum cell-free miR-214 was an independent prognostic indicator of overall survival for patients with glioma. Conclusions Serum cell-free miR-214 could serve as a promising noninvasive biomarker of glioma in tumor stratification, early diagnosis, and prognostic evaluation.
- Subjects :
- Male
Methyltransferase
Cell free
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Glioma
microRNA
Extracellular
medicine
Biomarkers, Tumor
Humans
Circulating MicroRNA
Noninvasive biomarkers
business.industry
Brain Neoplasms
medicine.disease
Prognosis
Gene Expression Regulation, Neoplastic
MicroRNAs
Isocitrate dehydrogenase
030220 oncology & carcinogenesis
Cancer research
Surgery
Female
Neurology (clinical)
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18788769
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- World neurosurgery
- Accession number :
- edsair.doi.dedup.....54415a25b4cbc2b020e53874eb6e4e35