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Folate-producing lactic acid bacteria reduce inflammation in mice with induced intestinal mucositis
- Source :
- Journal of Applied Microbiology. 125:1494-1501
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Aim: To evaluate two folate-producing strains, Streptococcus (Strep.) thermophilus CRL 808 and Strep. thermophilus CRL 415, against chemically induced mucositis in mice. Methods and Results: In vitro assays with Caco-2 cells were performed to evaluate the effect of the bacteria in the presence of 5-fluorouracil (5-FU). For in vivo studies, mice were daily injected with 5-FU to induce intestinal mucositis (IM) and orally administered with folate-producing strains during 6 days. Clinical symptoms, histological parameters and cytokine profiles were assessed. The results showed that Strep. thermophilus CRL 808 increased the cytotoxicity of 5-FU against Caco-2 cells. Administration of this strain in mice with chemically induced IM resulted in a reduction in diarrhoea score and restoration of the intestinal architecture. Cytokine analysis showed that the anti-inflammatory effect by the bacterium is not associated with an immune mechanism. Regarding Strep. thermophilus CRL 415, no improvements were observed in any of the parameters evaluated. Conclusion: The administration of the folate-producing Strep. thermophilus CRL 808 has the potential to prevent IM induced by 5-FU in mice. Significance and Impact of the Study: Folate-producing LAB could be used in chemotherapy patients to reduce the symptoms of IM, improve their nutritional status and increase the effectiveness of 5-FU. Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Ciencias de la Salud
medicine.disease_cause
Applied Microbiology and Biotechnology
law.invention
Mice
Probiotic
0302 clinical medicine
LACTIC ACID BACTERIA
Lactobacillales
law
Streptococcus thermophilus
Intestinal Mucosa
Streptococcus
PROBIOTIC
food and beverages
General Medicine
CHEMOTHERAPY
Otras Ciencias de la Salud
Intestines
Cytokine
030220 oncology & carcinogenesis
5-FLUOROURACIL
Cytokines
Fluorouracil
medicine.symptom
Biotechnology
Diarrhea
Mucositis
CIENCIAS MÉDICAS Y DE LA SALUD
Inflammation
Microbiology
03 medical and health sciences
Folic Acid
Immune system
INFLAMMATION
In vivo
medicine
Animals
Humans
Chemotherapy
business.industry
Probiotics
medicine.disease
030104 developmental biology
MUCOSITIS
Caco-2 Cells
business
FOLATE
Subjects
Details
- ISSN :
- 13645072
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Microbiology
- Accession number :
- edsair.doi.dedup.....54113afe558b856c7d06ecc661786fb7