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Dynamic assembly of Hda and the sliding clamp in the regulation of replication licensing
- Source :
- Nucleic Acids Research
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Regulatory inactivation of DnaA (RIDA) is one of the major regulatory mechanisms of prokaryotic replication licensing. In RIDA, the Hda–sliding clamp complex loaded onto DNA directly interacts with adenosine triphosphate (ATP)-bound DnaA and stimulates the hydrolysis of ATP to inactivate DnaA. A prediction is that the activity of Hda is tightly controlled to ensure that replication initiation occurs only once per cell cycle. Here, we determined the crystal structure of the Hda–β clamp complex. This complex contains two pairs of Hda dimers sandwiched between two β clamp rings to form an octamer that is stabilized by three discrete interfaces. Two separate surfaces of Hda make contact with the β clamp, which is essential for Hda function in RIDA. The third interface between Hda monomers occludes the active site arginine finger, blocking its access to DnaA. Taken together, our structural and mutational analyses of the Hda–β clamp complex indicate that the interaction of the β clamp with Hda controls the ability of Hda to interact with DnaA. In the octameric Hda–β clamp complex, the inability of Hda to interact with DnaA is a novel mechanism that may regulate Hda function.
- Subjects :
- DNA Replication
Models, Molecular
0301 basic medicine
DNA polymerase
Genome Integrity, Repair and Replication
DNA-binding protein
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
ATP hydrolysis
Genetics
Histone octamer
DNA Polymerase III
Adenosine Triphosphatases
DNA clamp
biology
Escherichia coli Proteins
DNA replication
DnaA
DNA-Binding Proteins
030104 developmental biology
chemistry
Mutation
Biophysics
biology.protein
bacteria
Protein Multimerization
Sequence Alignment
Adenosine triphosphate
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....5407817ca6ab7f3c6da056d57917bc8a