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The Oligo Fucoidan Inhibits Platelet-Derived Growth Factor-Stimulated Proliferation of Airway Smooth Muscle Cells
- Source :
- Marine Drugs, Vol 14, Iss 1, p 15 (2016), Marine Drugs; Volume 14; Issue 1; Pages: 15, Marine Drugs
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- In the pathogenesis of asthma, the proliferation of airway smooth muscle cells (ASMCs) is a key factor in airway remodeling and causes airway narrowing. In addition, ASMCs are also the effector cells of airway inflammation. Fucoidan extracted from marine brown algae polysaccharides has antiviral, antioxidant, antimicrobial, anticlotting, and anticancer properties; however, its effectiveness for asthma has not been elucidated thus far. Platelet-derived growth factor (PDGF)-treated primary ASMCs were cultured with or without oligo-fucoidan (100, 500, or 1000 µg/mL) to evaluate its effects on cell proliferation, cell cycle, apoptosis, and Akt, ERK1/2 signaling pathway. We found that PDGF (40 ng/mL) increased the proliferation of ASMCs by 2.5-fold after 48 h (p < 0.05). Oligo-fucoidan reduced the proliferation of PDGF-stimulated ASMCs by 75%–99% after 48 h (p < 0.05) and induced G1/G0 cell cycle arrest, but did not induce apoptosis. Further, oligo-fucoidan supplementation reduced PDGF-stimulated extracellular signal-regulated kinase (ERK1/2), Akt, and nuclear factor (NF)-κB phosphorylation. Taken together, oligo-fucoidan supplementation might reduce proliferation of PDGF-treated ASMCs through the suppression of ERK1/2 and Akt phosphorylation and NF-κB activation. The results provide basis for future animal experiments and human trials.
- Subjects :
- 0301 basic medicine
Platelet-derived growth factor
medicine.medical_treatment
Myocytes, Smooth Muscle
Pharmaceutical Science
Apoptosis
Pharmacology
Phaeophyta
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Polysaccharides
oligo-fucoidan
Drug Discovery
medicine
Animals
Humans
asthma
airway smooth muscle cells
Anti-Asthmatic Agents
Lung
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Protein kinase B
lcsh:QH301-705.5
Cells, Cultured
Cell Proliferation
Platelet-Derived Growth Factor
Dose-Response Relationship, Drug
biology
Cell growth
Growth factor
Cell Cycle
Cell cycle
Rats
030104 developmental biology
030228 respiratory system
chemistry
lcsh:Biology (General)
Immunology
biology.protein
Signal transduction
Platelet-derived growth factor receptor
Subjects
Details
- Language :
- English
- ISSN :
- 16603397
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....5401aafbd60da43516152b5f716e4636