Back to Search
Start Over
Salvia miltiorrhiza bunge increases estrogen level without side effects on reproductive tissues in immature/ovariectomized mice
- Source :
- Aging (Albany NY)
- Publication Year :
- 2016
- Publisher :
- Impact Journals LLC, 2016.
-
Abstract
- Salvia miltiorrhiza bunge (SM) is a popular herb for alleviating menopausal symptoms, although the scientific evidence of applying SM to estrogen replacement therapy is limited. In this study, we characterized the estrogenic activity of SM using in vivo models of immature and ovariectomized (OVX) mice and performed in vitro studies focusing on the estrogen receptor (ER) pathway for further molecular characterizations. SM treatments demonstrated significant estrogenic activity by promoting the development of uterus and vagina in immature mice, restoring the estrus cycle and reversing the atrophy of reproductive tissues in OVX mice, as well as increasing the expressions of ERα and ERβ at protein and mRNA level in the reproductive tissues. Meanwhile, SM significantly increased estradiol in serum, and decreased follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the circulation of immature and OVX mice. SM could stimulate the binding effect of ERα and ERβ, and significantly induce ERα/β-estrogen response element (ERE) luciferase reporter gene expression. All these activities were inhibited by the ER antagonist ICI182, 780. This study demonstrates SM exerts estrogenic effects by stimulating biosynthesis of estrogen and increasing ERs in target tissues without side effects on reproductive tissues and through ER-ERE-dependent pathway.
- Subjects :
- 0301 basic medicine
Salvia miltiorrhiza bunge
Aging
medicine.medical_specialty
medicine.drug_class
Ovariectomy
Uterus
Estrogen receptor
Estrous Cycle
Salvia miltiorrhiza
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
Internal medicine
medicine
Animals
Estrogen Receptor beta
Fulvestrant
Cell Proliferation
Estrous cycle
Estradiol
Chemistry
Plant Extracts
Estrogen Receptor alpha
estrogen receptor antagonist ICI182
estrogen receptors
Cell Biology
reproductive target tissue
estrogenic effect
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Estrogen
030220 oncology & carcinogenesis
Vagina
Ovariectomized rat
Female
Estrogen Receptor Antagonists
Luteinizing hormone
hormones, hormone substitutes, and hormone antagonists
Hormone
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....53fde844d8a4e455e511ddb2a9550fee