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A comparison of the reparative and angiogenic properties of mesenchymal stem cells derived from the bone marrow of BALB/c and C57/BL6 mice in a model of limb ischemia
- Source :
- Stem Cell Research & Therapy
- Publication Year :
- 2013
- Publisher :
- BioMed Central, 2013.
-
Abstract
- Introduction BALB/c mice and C57/BL6 mice have different abilities to recover from ischemia. C57/BL6 mice display increased vessel collateralization and vascular endothelial growth factor expression with a consequent rapid recovery from ischemia compared with BALB/c mice. Mesenchymal stem cells (MSCs) are one of the main cell types that contribute to the recovery from ischemia because, among their biological activities, they produce several proangiogenic paracrine factors and differentiate into endothelial cells. The objective of this study was to evaluate whether the MSCs of these two mouse strains have different inductive capacities for recovering ischemic limbs. Methods MSCs from these two strains were obtained from the bone marrow, purified and characterized before being used for in vivo experiments. Limb ischemia was surgically induced in BALB/c mice, and MSCs were injected on the fifth day. The evolution of limb necrosis was evaluated over the subsequent month. Muscle strength was assessed on the 30th day after the injection, and then the animals were sacrificed to determine the muscle mass and perform histological analyses to detect cellular infiltration, capillary and microvessel densities, fibrosis, necrosis and tissue regeneration. Results The MSCs from both strains promoted high level of angiogenesis similarly, resulting in good recovery from ischemia. However, BALB/c MSCs promoted more muscle regeneration (57%) than C57/BL6 MSCs (44%), which was reflected in the increased muscle strength (0.79 N versus 0.45 N). Conclusion The different genetic background of MSCs from BALB/c mice and C57/BL6 mice was not a relevant factor in promoting angiogenesis of limb ischemia, because both cells showed a similar angiogenic activity. These cells also showed a potential myogenic effect, but the stronger effect promoted by BALB/c MSCs indicates that the different genetic background of MSCs was more relevant in myogenesis than angiogesis.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Necrosis
Angiogenesis
Ischemia
Cell- and Tissue-Based Therapy
Medicine (miscellaneous)
Neovascularization, Physiologic
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cell therapy
BALB/c
chemistry.chemical_compound
Mice
Bone Marrow
medicine
Animals
Mice, Inbred BALB C
biology
Research
Mesenchymal stem cell
Endothelial Cells
Cell Biology
medicine.disease
biology.organism_classification
Hind-limb ischemia
Hindlimb
Vascular endothelial growth factor
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
chemistry
Commentary
Molecular Medicine
Mesenchymal stem cells
Bone marrow
Stem cell
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 17576512
- Volume :
- 4
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....53f80a31b9061bba4fa9b146c0e3656b