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Proteome analysis of aflatoxin B1-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) and functional identification of candidate protein peroxiredoxin II
- Source :
- Proteomics. 8(7)
- Publication Year :
- 2008
-
Abstract
- In order to explore the proteins responsible for hepatocellular carcinoma (HCC), aflatoxin B(1)-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) was analyzed with 2-DE and MS. By comparing HCC samples with their own precancerous biopsies and HCC-surrounding tissues, a group of candidate proteins that differentially expressed in HCC were obtained. Peroxiredoxin (Prx) II, one of the candidates with distinct alteration, was further investigated and validated. Western blot and RT-PCR assays confirmed the overexpression of Prx II in both tree shrew and human HCC tissues. RNA interference for silencing Prx II was employed subsequently to explore the function and underlying mechanism of Prx II on liver cancer cell line Hep3B. Results showed the cell proliferation and clone formation decreased obviously when Prx II expression was inhibited, while the flow cytometer analysis showed the percentage of cell apoptosis enhanced. Inhibition of Prx II expression also obviously increased the generation of ROS and malondialdehyde, both are the products from peroxidation. These results imply the important role of Prx II in hepatocarcinogenesis, possibly through its function in regulating peroxidation and hereby to provide a favorable microenvironment for cancer cell surviving and progressing.
- Subjects :
- Adult
Male
Aflatoxin B1
Carcinoma, Hepatocellular
Clone (cell biology)
Biology
medicine.disease_cause
Biochemistry
Mass Spectrometry
Liver Neoplasms, Experimental
Western blot
RNA interference
medicine
Animals
Humans
Electrophoresis, Gel, Two-Dimensional
Molecular Biology
Aged
Tupaia
medicine.diagnostic_test
Cell growth
Reverse Transcriptase Polymerase Chain Reaction
Liver Neoplasms
Peroxiredoxins
Middle Aged
Molecular biology
digestive system diseases
Apoptosis
Immunology
Cancer cell
Female
Carcinogenesis
Peroxiredoxin
Subjects
Details
- ISSN :
- 16159861
- Volume :
- 8
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Proteomics
- Accession number :
- edsair.doi.dedup.....53e39a1916a49eae07f47d773ceacf86