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A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The recurrence of new human cases of Middle East respiratory syndrome coronavirus (MERS-CoV) underscores the need for effective therapeutic countermeasures. Nonhuman primate models are considered the gold standard for preclinical evaluation of therapeutic countermeasures. However, MERS-CoV-induced severe respiratory disease in humans is associated with high viral loads in the lower respiratory tract, which may be difficult to achieve in nonhuman primate models. Considering this limitation, we wanted to ascertain the effectiveness of using a MERS-CoV infectious clone (icMERS-0) previously shown to replicate to higher titers than the wild-type EMC 2012 strain. We observed respiratory disease resulting from exposure to the icMERS-0 strain as measured by CT in rhesus monkeys with concomitant detection of virus antigen by immunohistochemistry. Overall, respiratory disease was mild and transient, resolving by day 30 post-infection. Although pulmonary disease was mild, these results demonstrate for the first time the utility of CT imaging to measure disease elicited by a MERS-CoV infectious clone system in nonhuman primate models.
- Subjects :
- 0301 basic medicine
Middle East respiratory syndrome coronavirus
Science
Clone (cell biology)
Disease
Virus Replication
medicine.disease_cause
Severity of Illness Index
Article
03 medical and health sciences
Virus antigen
Image Processing, Computer-Assisted
medicine
Animals
Humans
Lung
Multidisciplinary
business.industry
Respiratory disease
Viral Load
medicine.disease
Macaca mulatta
3. Good health
Disease Models, Animal
Titer
030104 developmental biology
medicine.anatomical_structure
Immunology
Middle East Respiratory Syndrome Coronavirus
Medicine
RNA, Viral
Coronavirus Infections
Tomography, X-Ray Computed
business
Viral load
Respiratory tract
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....53c9f2c8eaeef1e493bab20ad988df61