Back to Search
Start Over
Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves
- Source :
- Scientifica, Vol 2020 (2020), Scientifica
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.
- Subjects :
- Article Subject
Science
Ethyl acetate
03 medical and health sciences
Rutin
chemistry.chemical_compound
0404 agricultural biotechnology
Phenols
Gallic acid
030304 developmental biology
General Environmental Science
0303 health sciences
Traditional medicine
biology
fungi
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
chemistry
Phytochemical
Punica
Medicine
General Agricultural and Biological Sciences
Antibacterial activity
Research Article
Ellagic acid
Subjects
Details
- ISSN :
- 2090908X
- Volume :
- 2020
- Database :
- OpenAIRE
- Journal :
- Scientifica
- Accession number :
- edsair.doi.dedup.....53c29de3ae7481bfb8e6c7ce93eafb1e