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Corynebacterium diphtheriae putative tellurite-resistance protein (CDCE8392_0813) contributes to the intracellular survival in human epithelial cells and lethality of Caenorhabditis elegans
- Source :
- Memórias do Instituto Oswaldo Cruz, Memórias do Instituto Oswaldo Cruz., Vol 110, Iss 5, Pp 662-668 (2015), Memórias do Instituto Oswaldo Cruz., Iss 0, Pp 0-0 (2015), Memórias do Instituto Oswaldo Cruz, Volume: 110, Issue: 5, Pages: 662-668, Published: 24 JUN 2015
- Publication Year :
- 2015
- Publisher :
- Instituto Oswaldo Cruz, Ministério da Saúde, 2015.
-
Abstract
- Corynebacterium diphtheriae, the aetiologic agent of diphtheria, also represents a global medical challenge because of the existence of invasive strains as causative agents of systemic infections. Although tellurite (TeO32-) is toxic to most microorganisms, TeO32--resistant bacteria, including C. diphtheriae, exist in nature. The presence of TeO32--resistance (TeR) determinants in pathogenic bacteria might provide selective advantages in the natural environment. In the present study, we investigated the role of the putative TeR determinant (CDCE8392_813gene) in the virulence attributes of diphtheria bacilli. The disruption of CDCE8392_0813 gene expression in the LDCIC-L1 mutant increased susceptibility to TeO32- and reactive oxygen species (hydrogen peroxide), but not to other antimicrobial agents. The LDCIC-L1 mutant also showed a decrease in both the lethality of Caenorhabditis elegansand the survival inside of human epithelial cells compared to wild-type strain. Conversely, the haemagglutinating activity and adherence to and formation of biofilms on different abiotic surfaces were not regulated through the CDCE8392_0813 gene. In conclusion, the CDCE8392_813 gene contributes to the TeR and pathogenic potential of C. diphtheriae.
- Subjects :
- Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
Virulence Factors
Mutant
RC955-962
lcsh:QR1-502
Virulence
Microbial Sensitivity Tests
medicine.disease_cause
Microbiology
lcsh:Microbiology
HEp-2 cells
Bacterial Adhesion
Bacterial Proteins
Arctic medicine. Tropical medicine
tellurite-resistance
CDCE8392_0813 protein
medicine
Animals
Humans
Caenorhabditis elegans
Gene
Corynebacterium diphtheriae
biology
Biofilm
Pathogenic bacteria
Epithelial Cells
Articles
biology.organism_classification
QR1-502
Anti-Bacterial Agents
Tellurium
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 16788060 and 00740276
- Volume :
- 110
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz
- Accession number :
- edsair.doi.dedup.....53c0b40b83c5e00fcfd717e24128e072