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YAP induces cisplatin resistance through activation of autophagy in human ovarian carcinoma cells
- Source :
- OncoTargets and therapy
- Publication Year :
- 2016
- Publisher :
- Dove Press, 2016.
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Abstract
- Lan Xiao,1,* Xiao-Yan Shi,2,* Ying Zhang,1 Ying Zhu,1 Lin Zhu,3 Wang Tian,1 Bing-Kun Zhu,1 Zhao-Lian Wei11Department of Obstetrics and Gynecology, First Affiliated Hospital, Anhui Medical University, Hefei, 2Key Laboratory for Molecular Diagnosis of Hubei Province, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 3Obstetrics and Gynecology Hospital of Fudan University (Shanghai Red House Obstetrics and Gynecology Hospital), Shanghai, People’s Republic of China*These authors contributed equally tothis workObjective: To identify the role of YAP in cisplatin resistance in human ovarian cancer cells and in the regulation of autophagy in these cancer cells.Materials and methods: The cisplatin-sensitive OV2008 parental cell line and its cisplatin-resistant variant C13K were cultured. RNA interference was used to knock down the YAP gene. Accumulation of GFP-LC3 puncta was performed by fluorescence microscopy. The formation of autophagosomes was observed by transmission electron microscopy. Drug sensitivity was examined using CCK-8 assay, while apoptosis, the level of intracellular rhodamine 123 and lysosomal acidification were analyzed by fluorescence-activated cell sorting. Acid phosphatase activity was measured using an acid phosphatase-assay kit. Real-time polymerase chain reaction, Western blotting, and immunofluorescence detection were used to detect the protein and messenger RNA expression of YAP, YAP target genes, CCND1, cleaved PARP, and caspase3, Atg-3 and -5, and the LC3B protein.Results: YAP signaling may regulate cisplatin resistance in ovarian cancer cells by augmenting cellular autophagic flux. After knockdown of YAP-sensitized C13K cells to cisplatin by inducing a decrease in autophagy, YAP led to an increase in autophagy via enhancement of autolysosome degradation.Conclusion: YAP-mediated autophagy may play a protective role in cisplatin-resistant human ovarian cancer cells. Therefore, YAP-mediated autophagy should be explored as a new target for enhancing the efficacy of cisplatin against ovarian cancer and other types of malignancies. Keywords: YAP protein, autophagy, cisplatin resistance, ovarian carcinoma
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
autophagy
endocrine system diseases
business.industry
Cisplatin resistance
Autophagy
OncoTargets and Therapy
ovarian carcinoma
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
030220 oncology & carcinogenesis
Ovarian carcinoma
Internal medicine
Cancer cell
Ovarian cancer cells
Medicine
Pharmacology (medical)
YAP protein
cisplatin resistance
business
Original Research
Subjects
Details
- Language :
- English
- ISSN :
- 11786930
- Database :
- OpenAIRE
- Journal :
- OncoTargets and Therapy
- Accession number :
- edsair.doi.dedup.....5388734a303ad59c67a4dc772a062c1c