A New Biocatalytic Route to Enantiopure N-Carbamoyl Amino Acids by Fast Enzyme Screening

The enantioselective enzymatic deamidation of ( rac )- N -carbamoyl amino acid amides (Cbm-AA-NH 2 ) to enantiopure ( L )- N -carbamoyl amino acids (Cbm-AA-OH) is described for the first time. Via fast screening methods of biocatalysts several proteases like Chirazyme P1, Chirazyme P2 and Subtilisin were identified, which give conversions of up to 47% and >98% ee. This conversion is most productive on aliphatic and primary amino acids.