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Transcriptional Complementarity in Breast Cancer: Application to Detection of Circulating Tumor Cells

Authors :
Jiangchun Xu
Barbara K. Zehentner
Steven G. Reed
Cheryl Schmidt
Anthony Frudakis
Aristides Maltez Filho
Marcos Nolasco
John Jiang
Raymond L. Houghton
Xinqun Zhang
David H. Persing
David A. Molesh
Patrick C. Roche
Elizabeth A. Repasky
Davin C. Dillon
Roberto BadarĂ³
Source :
Molecular Diagnosis. 6:79-91
Publication Year :
2001
Publisher :
Springer Science and Business Media LLC, 2001.

Abstract

Background: We used a combination of genetic subtraction, silicon DNA microarray analysis, and quantitative PCR to identify tissue- and tumor-specific genes as diagnostic targets for breast cancer. Methods and Results: From a large number of candidate antigens, several specific subsets of genes were identified that showed concordant and complementary expression profiles. Whereas transcriptional profiling of mammaglobin resulted in the detection of 70% of tumors in a panel of 46 primary and metastatic breast cancers, the inclusion of three additional markers resulted in detection of all 46 specimens. Immunomagnetic epithelial cell enrichment of circulating tumor cells from the peripheral blood of patients with metastatic breast cancer, coupled with RT-PCR-based amplification of breast tumor-specific transcripts, resulted in the detection of anchorage-independent tumor cells in the majority of patients with breast cancer with known metastatic disease. Conclusion: Complementation of mammaglobin with three additional genes in RT-PCR increases the detection of breast cancers in tissue and circulating tumor cells.

Details

ISSN :
10848592
Volume :
6
Database :
OpenAIRE
Journal :
Molecular Diagnosis
Accession number :
edsair.doi.dedup.....536868724133fff38f031052eb885a3a
Full Text :
https://doi.org/10.1007/bf03262038