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Antioxidant and antibacterial activities, interfacial and emulsifying properties of the apo and holo forms of purified camel and bovine α-lactalbumin
- Source :
- International Journal of Biological Macromolecules, International Journal of Biological Macromolecules, Elsevier, 2020, 165, pp.205-213. ⟨10.1016/j.ijbiomac.2020.09.201⟩
- Publication Year :
- 2020
-
Abstract
- International audience; The antioxidant and antibacterial activities of camel and bovine α-lactalbumin (α-La) in both calcium-loaded (holo) and calcium-depleted (apo) forms were investigated and compared. Antioxidant assay showed that camel and bovine α-La exhibited significant Ferric-reducing antioxidant power (FRAP), ferrous iron-chelating activity (FCA) and antiradical activities especially in their apo form. Camel apo α-La also exhibited attractive antibacterial activities against Gram-negative bacteria (Pseudomonas aeruginosa) and against fungal pathogens species (Penicillium bilaiae, Aspergillus tamari and Aspergillus sclerotiorum). Likewise, emulsifying properties (emulsification ability (EAI) and stability (ESI) indexes) and the surface characteristics (surface hydrophobicity, ζ-potential and interfacial tension) of the α-La were assessed. Maximum EAI were found at pH 7.0, with higher EAI values for the camel apo α-La (EAI ~19.5 m2/g). This behavior was explained by its relative high surface hydrophobicity and its greater efficiency to reduce the surface tension at the oil-water interface. Furthermore, emulsions were found to be more stable at pH 7.0 compared to pH 5.0 (ESI ~50%) due to the higher electrostatic repulsive forces between oil droplets at pH 7.0 in consistence with the ζ-potential results. This study concluded that the camel apo α-La has antibacterial, antioxidant, and emulsifying properties in agricultural and food industries.
- Subjects :
- Antioxidant
Camelus
medicine.medical_treatment
02 engineering and technology
Biochemistry
Antioxidants
Ferrous
03 medical and health sciences
Apoenzymes
Antioxidant activity
Structural Biology
medicine
[CHIM]Chemical Sciences
Animals
Penicillium bilaiae
Food science
Molecular Biology
Purification
030304 developmental biology
Lactalbumin
0303 health sciences
biology
Chemistry
Penicillium
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Aspergillus sclerotiorum
Anti-Bacterial Agents
Emulsification
Aspergillus
13. Climate action
Aspergillus tamari
Camel alpha-lactalbumin
Cattle
Emulsions
Antibacterial activity
0210 nano-technology
Holoenzymes
Hydrophobic and Hydrophilic Interactions
Bacteria
Subjects
Details
- ISSN :
- 18790003 and 01418130
- Volume :
- 165
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....53642dd384dad77646afa037b40311fc