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Impact of Fresh Table Grape Intake on Circulating microRNAs Levels in Healthy Subjects: A Significant Modulation of Gastrointestinal Cancer‐Related Pathways
- Source :
- Molecular Nutrition & Food Research. 65:2100428
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- SCOPE The study aims to investigate the effects of fresh table grape consumption in healthy subjects on circulating levels of the most common human microRNAs (miRNAs). The regulatory network governed by these modulated miRNAs is also investigated. METHODS AND RESULTS Autumn Royal table grape, used in this study, is chosen for its high polyphenolic content and antioxidant properties. The study is a randomized controlled trial, in which 40 consecutive subjects are recruited on a voluntary basis and randomly assigned to two groups of the study, the control group, receiving only dietary recommendations and a grape group receiving a daily dose of 5 g of fresh table grape per kg of body weight for 21 days. All analyses are performed at baseline and after 21 days of dietary treatment. Circulating miRNAs levels are detected by Real-Time quantitative PCR (RT-qPCR) followed by bioinformatic functional analysis. The study identifies 20 circulating miRNAs differentially expressed in healthy subjects after grape intake, and in particular, 18 of 20 are down-regulated and 2 are up-regulated. CONCLUSION The dietary intake of table grape affects circulating miRNAs levels in healthy subjects, particularly the miRNAs related to pathways involved in counteracting cancer development, including gastrointestinal cancers.
- Subjects :
- business.industry
Dietary intake
Table grape
Healthy subjects
food and beverages
Physiology
medicine.disease
Healthy Volunteers
law.invention
MicroRNAs
Circulating MicroRNA
Real-time polymerase chain reaction
Nutrigenomics
Randomized controlled trial
law
Humans
Medicine
Vitis
Gastrointestinal cancer
business
Gastrointestinal Neoplasms
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 16134133 and 16134125
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Molecular Nutrition & Food Research
- Accession number :
- edsair.doi.dedup.....534a5773abba23e37156bfb0c8b8d573