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Serrulatane Diterpenoid from Eremophila neglecta Exhibits Bacterial Biofilm Dispersion and Inhibits Release of Pro-inflammatory Cytokines from Activated Macrophages
- Source :
- Journal of natural products. 78(12)
- Publication Year :
- 2015
-
Abstract
- The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity of a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from the Australian medicinal plant Eremophila neglecta. Biofilm breakup activity of compound 1 on established Staphylococcus epidermidis and Staphylococcus aureus biofilms was compared to the antiseptic chlorhexidine and antibiotic levofloxacin. In a time-course study, 1 was deposited onto polypropylene mesh to mimic a wound dressing and tested for biofilm removal. The ex-vivo cytotoxicity and effect on lipopolysaccharide-induced pro-inflammatory cytokine release were studied in mouse primary bone-marrow-derived macrophage (BMDM) cells. Compound 1 was effective in dispersing 12 h pre-established biofilms with a 7 log10 reduction of viable bacterial cell counts, but was less active against 24 h biofilms (approximately 2 log10 reduction). Compound-loaded mesh showed dosage-dependent biofilm-removing capability. In addition, compound 1 displayed a significant inhibitory effect on tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) secretion from BMDM cells, but interleukin-1 beta (IL-1β) secretion was not significant. The compound was not cytotoxic to BMDM cells at concentrations effective in removing biofilm and lowering cytokine release. These findings highlight the potential of this serrulatane diterpenoid to be further developed for applications in wound management. Refereed/Peer-reviewed
- Subjects :
- Lipopolysaccharides
Staphylococcus aureus
Cell Survival
medicine.medical_treatment
Interleukin-1beta
Pharmaceutical Science
serrulatane diterpenoid
Levofloxacin
Microbial Sensitivity Tests
medicine.disease_cause
immune response
biofilm
Analytical Chemistry
Proinflammatory cytokine
Microbiology
Mice
Eremophila Plant
Staphylococcus epidermidis
Drug Discovery
medicine
Animals
Secretion
wound management
Cytotoxicity
Pharmacology
Plants, Medicinal
biology
Dose-Response Relationship, Drug
Molecular Structure
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophages
Organic Chemistry
Biofilm
Australia
Staphylococcal Infections
biology.organism_classification
Anti-Bacterial Agents
Cytokine
Complementary and alternative medicine
Biofilms
Molecular Medicine
Cytokines
Tumor necrosis factor alpha
Diterpenes
Scrophulariaceae
Subjects
Details
- ISSN :
- 15206025
- Volume :
- 78
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of natural products
- Accession number :
- edsair.doi.dedup.....53451a0f4f3391fdce7554e67790e926