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Effects of endopeptidase inhibition on the relaxation response of isolated human penile erectile tissue to vasoactive peptides
- Source :
- Andrologia. 48(10)
- Publication Year :
- 2016
-
Abstract
- Peptides, such as CNP, CGRP and VIP, are involved in the function of male penile erectile tissue. Tissue levels of said peptides are controlled by the endopeptidase enzymes. Theoretically, the inhibition of the degradation of CNP, CGRP and/or VIP should result in an enhancement in penile smooth muscle relaxation. The effects were investigated of CNP or VIP (0.1 nm-1 μm), without and following pre-exposure of the tissue to a threshold concentration of the endopeptidase inhibitor KC 12615 (10 μm, for 20 min), on the reversion of tension induced by means of electrical field stimulation. Drug effects on the production of cyclic AMP/GMP were also evaluated. Neither KC 12615, CNP and VIP nor the combination of CNP plus KC 12615 or VIP plus KC 12615 increased the response of the tissue to EFS. While no effects were observed of a pre-exposure of the tissue to KC 12615 on the production of cyclic AMP in the presence of VIP, an enhancement was registered in the accumulation of cyclic AMP in the presence of CNP plus KC 12615. Further studies are indicated to investigate whether endopeptidase inhibitors might tend to be more effective in tissues affected by a decreased local production of vasoactive peptides.
- Subjects :
- Adult
Male
medicine.medical_specialty
Urology
Muscle Relaxation
Vasoactive intestinal peptide
030232 urology & nephrology
Reversion
Erectile tissue
Calcitonin gene-related peptide
03 medical and health sciences
Young Adult
0302 clinical medicine
Endocrinology
Internal medicine
medicine
Cyclic AMP
Humans
Protease Inhibitors
Cyclic GMP
chemistry.chemical_classification
Chemistry
Muscle, Smooth
Natriuretic Peptide, C-Type
General Medicine
Middle Aged
Endopeptidase
Electric Stimulation
Muscle relaxation
medicine.anatomical_structure
Enzyme
030220 oncology & carcinogenesis
medicine.symptom
Muscle contraction
Muscle Contraction
Penis
Vasoactive Intestinal Peptide
Subjects
Details
- ISSN :
- 14390272
- Volume :
- 48
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Andrologia
- Accession number :
- edsair.doi.dedup.....5340fea2cad30e488062406c05febf14