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Elucidating the Inability of Functionalized Nanoparticles to Cross the Blood–Brain Barrier and Target Specific Cells in Vivo
- Source :
- ACS Applied Materials & Interfaces. 11:22085-22095
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- The adsorption of serum proteins on the surface of nanoparticles (NPs) delivered into a biological environment has been known to alter NP surface properties and consequently their targeting efficiency. In this paper, we use random copolymer (p(HEMA- ran-GMA))-based NPs synthesized using 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA). We show that serum proteins bind to the NP and that functionalization with antibodies and peptides designed to facilitate NP passage across the blood-brain barrier (BBB) to bind specific cell types is ineffective. In particular, we use systematic in vitro and in vivo analyses to demonstrate that p(HEMA- ran-GMA) NPs functionalized with HIV-1 trans-activating transcriptor peptide (known to cross the BBB) and α neural/glial antigen 2 (NG2) (known for targeting oligodendrocyte precursor cells (OPCs)), individually and in combination, do not specifically target OPCs and are unable to cross the BBB, likely due to the serum protein binding to the NPs.
- Subjects :
- Male
Glycidyl methacrylate
Materials science
Polymers
Peptide
02 engineering and technology
010402 general chemistry
Blood–brain barrier
01 natural sciences
chemistry.chemical_compound
Antigen
In vivo
medicine
Animals
General Materials Science
Oligodendrocyte Precursor Cells
chemistry.chemical_classification
Microscopy, Confocal
technology, industry, and agriculture
Biological Transport
021001 nanoscience & nanotechnology
Blood proteins
In vitro
Rats
0104 chemical sciences
medicine.anatomical_structure
nervous system
chemistry
Blood-Brain Barrier
Drug delivery
Biophysics
Epoxy Compounds
Methacrylates
Nanoparticles
Female
0210 nano-technology
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....5337caedbe7319420717ff137652004e