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Establishment and characterization of two epithelial tumor cell lines (HNE-1 and HONE-1) latently infected with Epstein-Barr virus and derived from nasopharyngeal carcinomas
- Source :
- International journal of cancer. 45(1)
- Publication Year :
- 1990
-
Abstract
- Two epithelial tumor cell lines were established from biopsy specimens of 2 nasopharyngeal carcinomas (NPC) and designated HNE-1 and HONE-1. Uncloned HNE-1 cells were found to be Epstein-Barr virus (EBV) DNA-positive when examined by Southern blot analysis up to passage 35, after which the EBV genome could no longer be detected. A similar loss of EBV DNA took place in uncloned HONE-1 cells. However, HONE-1 clone 40 cells are still EBV DNA-positive up to passage 42 thus far and cell cultures contain 85-90% EBV nuclear antigen (EBNA)-positive cells. The HNE-1 cell line has been passaged more than 100 times and the uncloned HONE-1 cells more than 90 times. The tumorigenicity of the HNE-1 and HONE-1 cells was demonstrated by tumor induction in nude mice. Karyotypic analysis of the HNE-1 cells demonstrated an aneuploidy with a modal chromosomal number of 74 at passages 5 and 101 at passage 20; 18 marker chromosomes were identified. We have continued to map the EBV genome latently associated with the HNE-1 and HONE-1 cells using the Bam HI, EcoRI or Hind III restriction enzymes. Using EcoRI fragments A-K as probes, we found that HNE-1 EBV DNA is different from B95-8 and HR-1 EBV DNA in the EcoRI-C region. The Bam HI map for HONE-1 EBV DNA is very similar to the B95-8 map; it contains the Bam HI-Y fragment but without Bam HI B' and WI'. Differences were observed between HONE-1 EBV DNA and B95-8 DNA using the Hind III restriction enzyme. There was no evidence of spontaneous expression of the latent EBV genome in HNE-1 cells, and attempts to induce replication of the latent EBV genome and rescue infectious virus have failed, suggesting a tightly restricted virus genome.
- Subjects :
- Gene Expression Regulation, Viral
Male
Cancer Research
Herpesvirus 4, Human
Genes, Viral
Restriction Mapping
Mice, Nude
medicine.disease_cause
Virus Replication
Virus
Epithelium
Cell Line
Mice
Restriction map
hemic and lymphatic diseases
medicine
Tumor Cells, Cultured
Gammaherpesvirinae
Animals
Humans
Gene
Southern blot
biology
Nasopharyngeal Neoplasms
biology.organism_classification
Epstein–Barr virus
Virology
Restriction enzyme
Blotting, Southern
Tumor Virus Infections
Oncology
Karyotyping
Female
Carcinogenesis
Subjects
Details
- ISSN :
- 00207136
- Volume :
- 45
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of cancer
- Accession number :
- edsair.doi.dedup.....533715236487baa6e1bf95140079c2d4