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Involvement of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the pathogenesis of granulomatous colitis in rats

Authors :
Shigeaki Nagao
A. Kawaguchi
S. Miura
Shingo Kato
Hiroshi Nagata
T. Iizuka
H. Ishii
T. Miyahara
Kazurou Itoh
Atsuhiro Iwai
Ryota Hokari
Kouji Matsuzaki
Masayuki Miyasaka
Eiichi Sekizuka
Source :
Clinical and experimental immunology. 126(2)
Publication Year :
2001

Abstract

SummaryAlthough increased expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) has been demonstrated in inflammatory sites of various diseases, its role in colitis remains unknown. In this study, we examined whether MAdCAM-1 is involved in the pathogenesis of granulomatous colitis induced by peptidoglycan-polysaccharide (PG-PS). Experimental colitis was induced by intramural injection of PG-PS to rat colon. After 3 weeks the colon was removed and the mucosal inflammation was assessed. The area of MAdCAM-1-positive venules and the subsets of infiltrating cells were determined in colonic mucosa by immunohistochemistry. In another experiment, monoclonal antibody against MAdCAM-1 was administered intraperitoneally to examine its attenuating effect on colitis. The intramural injection of PG-PS induced significant colonic inflammation with granuloma formation. The submucosa was drastically thickened with the infiltration of CD4 positive lymphocytes and ED-1 positive macrophages. Intense MAdCAM-1 expression was observed on endothelium of the submucosal venules in inflamed mucosa. Administration of anti-MAdCAM-1 antibody significantly attenuated the PG-PS-induced colonic damage and cell infiltration. Enhanced expression of MAdCAM-1 was demonstrated in venular endothelium of the inflamed colon in PG-PS-induced colitis. The attenuating effect of anti-MAdCAM-1 suggests the importance of the MAdCAM-1-dependent process in the formation of chronic granulomatous colitis.

Details

ISSN :
00099104
Volume :
126
Issue :
2
Database :
OpenAIRE
Journal :
Clinical and experimental immunology
Accession number :
edsair.doi.dedup.....532fcc2c02efc2a017fab665ffbbdd0d