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Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo
- Source :
- Parasitology Research
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Coccidiosis triggered by Eimeria tenella is accompanied by haemorrhagic caecum and high morbidity. Vaccines are preferable choices to replace chemical drugs against coccidiosis. Surface antigens of apicomplexan parasites can adhere to host cells during the infection process. Therefore, truncated fragments coding E. tenella surface antigen 16 (EtSAG16) and 22 (EtSAG22) were cloned into pET-28a prokaryotic vector to express recombinant protein 16 (rEtSAG16) and 22 (rEtSAG22), respectively. Likewise, pEGFP-N1-EtSAG16 and pEGFP-N1-EtSAG22 plasmids were constructed using pEGFP-N1 eukaryotic vector. Further, pEGFP-N1-EtSAG4-16-22 multiple gene plasmid carrying EtSAG4, 16 and 22 were designed as cocktail vaccines to study integral immunoprotective effects. Western blot and RT-PCR (reverse transcription) assay were performed to verify expressions of EtSAG16 and 22 genes. Immunoprotective effects of recombinant protein or DNA vaccine were evaluated using different doses (50 or 100 μg) in vivo. All chickens in the vaccination group showed higher cytokine concentration (IFN-γ and IL-17), raised IgY antibody level, increased weight gain, lower caecum lesion score and reduced oocyst shedding compared with infection control groups (p < 0.05). The highest anticoccidial index (ACI) value 173.11 was from the pEGFP-N1-EtSAG4-16-22 plasmid (50 μg) group. In conclusion, EtSAG16 and 22 might be alternative candidate genes for generating vaccines against E. tenella infection.
- Subjects :
- Protozoan Vaccines
DNA vaccine
Recombinant protein
030231 tropical medicine
Protozoan Proteins
Eimeria
030308 mycology & parasitology
DNA vaccination
law.invention
Microbiology
03 medical and health sciences
Immunogenicity, Vaccine
0302 clinical medicine
Plasmid
Antigen
law
In vivo
Vaccines, DNA
medicine
Animals
Poultry Diseases
Vaccines, Synthetic
0303 health sciences
Cocktail vaccine
Immunoprotective
General Veterinary
biology
Coccidiosis
Oocysts
General Medicine
biology.organism_classification
medicine.disease
Recombinant Proteins
Vaccination
Infectious Diseases
Protozoology - Original Paper
Insect Science
Antigens, Surface
Recombinant DNA
Cytokines
Parasitology
Chickens
Eimeria tenella
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....5325fb9fd7b21abdd9a575aac4903d7f