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TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 284:G604-G616
- Publication Year :
- 2003
- Publisher :
- American Physiological Society, 2003.
-
Abstract
- We investigated which transient receptor potential (TRP) channel is responsible for the nonselective cation channel (NSCC) activated by carbachol (CCh) in murine stomach with RT-PCR and the electrophysiological method. All seven types of TRP mRNA were detected in murine stomach with RT-PCR. When each TRP channel was expressed, the current-voltage relationship of mTRP5 was most similar to that recorded in murine gastric myocytes. mTRP5 showed a conductance order of Cs+> K+> Na+, similar to that in the murine stomach. With 0.2 mM GTPĪ³S in the pipette solution, the current was activated transiently in both NSCC in the murine stomach and the expressed mTRP5. Both NSCC activated by CCh in murine stomach and mTRP5 were inhibited by intracellularly applied anti-Gq/11antibody, PLC inhibitor U-73122, IICR inhibitor 2-aminoethoxydiphenylborate, and nonspecific cation channel blockers La3+and flufenamate. There were two other unique properties. Both the native NSCC and mTRP5 were activated by 1-oleoyl-2-acetyl-sn-glycerol. Without the activation of NSCC by CCh, the NSCC in murine stomach was constitutively active like mTRP5. From the above results, we suggest that mTRP5 might be a candidate for the NSCC activated by ACh or CCh in murine stomach.
- Subjects :
- Male
Carbachol
Physiology
Gene Expression
Stimulation
CHO Cells
GTP-Binding Protein alpha Subunits, Gi-Go
Muscarinic Agonists
TRPC5
Antibodies
Membrane Potentials
Diglycerides
Mice
Transient receptor potential channel
Cations
Cricetinae
Physiology (medical)
Muscarinic acetylcholine receptor
medicine
Animals
Cation Transport Proteins
TRPC Cation Channels
Acetylcholine receptor
Mice, Inbred ICR
Hepatology
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Anti-Inflammatory Agents, Non-Steroidal
Stomach
Gastroenterology
Muscle, Smooth
Heterotrimeric GTP-Binding Proteins
Flufenamic Acid
Electrophysiology
Biochemistry
Gastric Mucosa
Biophysics
GTP-Binding Protein alpha Subunits, Gq-G11
Calcium
Female
Calcium Channels
Acetylcholine
medicine.drug
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 284
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....5324f598c0629e97a6254bb4dc74972c