Back to Search
Start Over
Alginate Nanoparticles Enhance Anti-Clostridium perfringens Activity of the Leaderless Two-Peptide Enterocin DD14 and Affect Expression of Some Virulence Factors
- Source :
- Probiotics and Antimicrobial Proteins, Probiotics and Antimicrobial Proteins, 2021, 13 (4), pp.1213-1227. ⟨10.1007/s12602-020-09730-y⟩
- Publication Year :
- 2020
-
Abstract
- International audience; Here, we report a novel approach to improve the anti-Clostridium perfringens activity of the leaderless two-peptide enterocin 14 (EntDD14), produced by Enterococcus faecalis 14. This strategy consists of loading EntDD14 onto alginate nanoparticles (Alg NPs), which are made of a safe polymer. The resulting formulation (EntDD14/Alg NPs) was able to reduce up to four times the minimum inhibitory concentration (MIC) of EntDD14 against C. perfringens pathogenic strains isolated from a chicken affected by necrotic enteritis (NE). Interestingly, this formulation remained active under conditions mimicking the human and chicken gastric tract. Assays conducted to establish the impact of this formulation on the intestinal epithelial cell line Caco-2 and the human colorectal adenocarcinoma cell line HT29 revealed the absence of cytotoxicity of both free-EntDD14 and EntDD14 loaded onto the alginate nanoparticles (EntDD14/Alg NPs) against the aforementioned eukaryotic cells, after 24 h of contact. Notably, EntDD14 and EntDD14/Alg NPs, both at a sub-inhibitory concentration, affected the expression of genes coding for clostridial toxins such as toxin α, enteritis B-like toxin, collagen adhesion protein and thiol-activated cytolysin. Further, expression of these genes was significantly down-regulated following the addition of EntDD14/Alg NPs, but not affected upon addition of EntDD14 alone. This study revealed that adsorption of EntDD14 onto Alg NPs leads to a safe and active formulation (EntDD14/Alg NPs) capable of affecting the pathogenicity of C. perfringens. This formulation could therefore be used in the poultry industry as a novel approach to tackle NE.
- Subjects :
- 0301 basic medicine
Bridged-Ring Compounds
Alginates
Clostridium perfringens
Virulence Factors
Cytotoxicity
030106 microbiology
Virulence
medicine.disease_cause
Microbiology
Enterococcus faecalis
[SPI]Engineering Sciences [physics]
03 medical and health sciences
Minimum inhibitory concentration
medicine
Animals
Humans
Alginate nanoparticles
Expression of toxin genes
Molecular Biology
Necrotic enteritis
Enterocin DD14
biology
Toxin
Chemistry
biology.organism_classification
Enteritis
030104 developmental biology
Cell culture
Molecular Medicine
Nanoparticles
Cytolysin
Caco-2 Cells
Peptides
Chickens
HT29 Cells
Subjects
Details
- ISSN :
- 18671314 and 18671306
- Volume :
- 13
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Probiotics and antimicrobial proteins
- Accession number :
- edsair.doi.dedup.....530e666a26a80d3894af2c26585b4094