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Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
- Source :
- PLoS ONE, Vol 13, Iss 6, p e0198739 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, and industry. In this study, we performed systematic liquid chromatography–mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts. Partial least-squares discriminant analysis of LC-MS datasets for the extracts of 34 plant species belonging to the families Aceraceae, Asteraceae, and Rosaceae showed that these species were clustered according to their respective phylogenies. In particular, seven Aceraceae species were clearly demarcated with higher average antioxidant activities, rationalizing their application for bioconversion studies. On the basis of further evaluation of the interspecies variability of metabolic profiles and antioxidant activities among Aceraceae family plants, we found that Acer tataricum (TA) extracts were clearly distinguished from those of other species, with a higher relative abundance of tannin derivatives. Further, we detected a strong positive correlation between most tannin derivatives and the observed higher antioxidant activities. Following Aspergillus oryzae-mediated fermentative bioconversion of Acer plant extracts, we observed a time-correlated (0–8 days) linear increase in antioxidant phenotypes for all species, with TA having the highest activity. Temporal analysis of the MS data revealed tannin bioconversion mechanisms with a relatively higher abundance of gallic acid (m/z 169) accumulated at the end of 8 days, particularly in TA. Similarly, quercetin precursor (glycoside) metabolites were also transformed to quercetin aglycones (m/z 301) in most Acer plant extracts. The present study underscores the efficacy of fermentative bioconversion strategies aimed at enhancing the quality and availability of bioactive metabolites from plant extracts.
- Subjects :
- Metabolic Processes
0301 basic medicine
Antioxidant
Bioconversion
Aspergillus oryzae
Applied Microbiology
medicine.medical_treatment
lcsh:Medicine
Biochemistry
01 natural sciences
Antioxidants
Mass Spectrometry
chemistry.chemical_compound
Drug Metabolism
Metabolites
Medicine and Health Sciences
Tannin
Food science
Gallic acid
lcsh:Science
Flowering Plants
chemistry.chemical_classification
Multidisciplinary
biology
Eukaryota
food and beverages
Plants
Chemistry
Aceraceae
Physical Sciences
Metabolome
Quercetin
Research Article
Biotechnology
Acer
Microbiology
Industrial Microbiology
03 medical and health sciences
Metabolomics
Phenols
Gallic Acid
medicine
Pharmacokinetics
Pharmacology
Plant Extracts
lcsh:R
010401 analytical chemistry
Organisms
Chemical Compounds
Biology and Life Sciences
Glycoside
biology.organism_classification
0104 chemical sciences
Metabolism
030104 developmental biology
chemistry
lcsh:Q
Tannins
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....52f9a8b4c2f894527bed19be33866f54