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Salidroside inhibits migration, invasion and angiogenesis of MDA‑MB�231 TNBC cells by regulating EGFR/Jak2/STAT3 signaling via MMP2
- Source :
- International Journal of Oncology.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- The major hallmarks of tumor progression are angiogenesis, migration and metastasis. Among the components of Rhodiola rosea, salidroside (p‑hydroxyphenethyl-β‑d-glucoside) is one of the most potent, and is present in all Rhodiola species. Recent data have revealed the anticancer effects of salidroside; however, the mechanism underlying its ability to inhibit tumor angiogenesis remains unknown. The present study aimed to analyze how salidroside affects major factors involved in breast cancer, and to elucidate its ability to inhibit angiogenesis and invasion. Signal transducer and activator of transcription 3 (STAT3) is a marker for tumor angiogenesis and migration, which interacts with matrix metalloproteinases (MMPs). Specifically, MMPs act as a downstream target for STAT3. Using western blotting and reverse transcription-quantitative polymerase chain reaction analysis, the present study demonstrated that treatment of MDA‑MB 231 triple-negative breast cancer (TNBC) cells with salidroside led to inhibition of invasion and migration markers, and of STAT3 signaling. Furthermore, in vitro angiogenesis analyses in human umbilical vein endothelial cells confirmed the anti-angiogenic activity of salidroside. An electrophoretic mobility shift assay also demonstrated that salidroside may inhibit the DNA-binding activity of STAT3, preventing STAT3 from binding to a novel binding site of the MMP2 gene promoter. In conclusion, the present results demonstrated that salidroside may downregulate the STAT3 signaling pathway, and inhibit cell viability, migration and invasion through MMPs in breast cancer cells.
- Subjects :
- STAT3 Transcription Factor
0301 basic medicine
Cancer Research
MMP2
Cell Survival
Angiogenesis
Angiogenesis Inhibitors
Triple Negative Breast Neoplasms
Matrix metalloproteinase
Biology
Metastasis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Glucosides
Phenols
Cell Movement
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
STAT3
Salidroside
Janus Kinase 2
medicine.disease
Antineoplastic Agents, Phytogenic
ErbB Receptors
Gene Expression Regulation, Neoplastic
030104 developmental biology
Oncology
chemistry
Tumor progression
030220 oncology & carcinogenesis
STAT protein
Cancer research
biology.protein
Matrix Metalloproteinase 2
Female
Signal Transduction
Subjects
Details
- ISSN :
- 17912423 and 10196439
- Database :
- OpenAIRE
- Journal :
- International Journal of Oncology
- Accession number :
- edsair.doi.dedup.....52f777faa7576647b25e9f0a046d77e3
- Full Text :
- https://doi.org/10.3892/ijo.2018.4430