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Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps
- Source :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.M112.365924⟩, Journal of Biological Chemistry, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.M112.365924⟩, Journal of Biological Chemistry, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.m112.365924⟩, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.m112.365924⟩
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- International audience; Hepatitis C virus (HCV) particles assemble along the very low density lipoprotein pathway and are released from hepatocytes as entities varying in their degree of lipid and apolipoprotein (apo) association as well as buoyant densities. Little is known about the cell entry pathway of these different HCV particle subpopulations, which likely occurs by regulated spatiotemporal processes involving several cell surface molecules. One of these molecules is the scavenger receptor BI (SR-BI), a receptor for high density lipoprotein that can bind to the HCV glycoprotein E2. By studying the entry properties of infectious virus subpopulations differing in their buoyant densities, we show that these HCV particles utilize SR-BI in a manifold manner. First, SR-BI mediates primary attachment of HCV particles of intermediate density to cells. These initial interactions involve apolipoproteins, such as apolipoprotein E, present on the surface of HCV particles, but not the E2 glycoprotein, suggesting that lipoprotein components in the virion act as host-derived ligands for important entry factors such as SR-BI. Second, we found that in contrast to this initial attachment, SR-BI mediates entry of HCV particles independent of their buoyant density. This function of SR-BI does not depend on E2/SR-BI interaction but relies on the lipid transfer activity of SR-BI, probably by facilitating entry steps along with other HCV entry co-factors. Finally, our results underscore a third function of SR-BI governed by specific residues in hypervariable region 1 of E2 leading to enhanced cell entry and depending on SR-BI ability to bind to E2.
- Subjects :
- Apolipoprotein E
Very low-density lipoprotein
MESH: Virus Internalization
Hepacivirus
medicine.disease_cause
Biochemistry
MESH: Protein Structure, Tertiary
Mice
0302 clinical medicine
Viral Envelope Proteins
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Animals
MESH: Hepacivirus
Receptor
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
chemistry.chemical_classification
0303 health sciences
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
virus diseases
Scavenger Receptors, Class B
MESH: Apolipoproteins E
3. Good health
Cell biology
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
lipids (amino acids, peptides, and proteins)
030211 gastroenterology & hepatology
MESH: Cell Line, Tumor
MESH: Rats
Viral protein
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Biology
Microbiology
Apolipoproteins E
03 medical and health sciences
Cell Line, Tumor
medicine
Animals
Humans
Scavenger receptor
MESH: Mice
Molecular Biology
030304 developmental biology
MESH: Humans
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Cell Biology
Virus Internalization
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
MESH: Scavenger Receptors, Class B
digestive system diseases
Protein Structure, Tertiary
Rats
chemistry
MESH: Viral Envelope Proteins
Glycoprotein
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Lipoprotein
Subjects
Details
- ISSN :
- 00219258 and 1083351X
- Volume :
- 287
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....52e1a22ec888ffbb041a49a3abb40db5
- Full Text :
- https://doi.org/10.1074/jbc.m112.365924