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Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps

Authors :
Marlène Dreux
Ophélia Granio
Thomas F. Baumert
Ralf Bartenschlager
Maryse Guerin
Christelle Granier
François-Loïc Cosset
François Penin
Mirjam B. Zeisel
Viet Loan Dao Thi
Dimitri Lavillette
Jimmy Mancip
Virologie humaine
École normale supérieure - Lyon (ENS Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM)
Department of Medicine II
University of Freiburg [Freiburg]
Dyslipidémies, inflammation et athérosclérose dans les maladies métaboliques
Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Institut de biologie et chimie des protéines [Lyon] (IBCP)
Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Department of Molecular Virology (DMV)
Universität Heidelberg [Heidelberg]
Plate-forme de R&D - Puces à Cellules Transfectées (PCT)
Cancéropôle du Grand-Est (CGE)-Centre Européen de Biologie et de Génomique Structurale (CEBGS)
Naiglin, Laurence
Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR)
Centre International de Recherche en Infectiologie (CIRI)
École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Interaction virus-hôte et maladies du foie
Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Institut National de la Santé et de la Recherche Médicale (INSERM)
École normale supérieure de Lyon (ENS de Lyon)-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM)
Bases moléculaires et structurales des systèmes infectieux (BMSSI)
Universität Heidelberg [Heidelberg] = Heidelberg University
Virus, mmunité innée et trafic vésiculaire - Vesicular trafficking, innate response and viruses (VIV)
Centre International de Recherche en Infectiologie - UMR (CIRI)
École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Référent HAL, CIRI
Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Source :
Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.M112.365924⟩, Journal of Biological Chemistry, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.M112.365924⟩, Journal of Biological Chemistry, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.m112.365924⟩, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31242-31257. ⟨10.1074/jbc.m112.365924⟩
Publication Year :
2012
Publisher :
Elsevier BV, 2012.

Abstract

International audience; Hepatitis C virus (HCV) particles assemble along the very low density lipoprotein pathway and are released from hepatocytes as entities varying in their degree of lipid and apolipoprotein (apo) association as well as buoyant densities. Little is known about the cell entry pathway of these different HCV particle subpopulations, which likely occurs by regulated spatiotemporal processes involving several cell surface molecules. One of these molecules is the scavenger receptor BI (SR-BI), a receptor for high density lipoprotein that can bind to the HCV glycoprotein E2. By studying the entry properties of infectious virus subpopulations differing in their buoyant densities, we show that these HCV particles utilize SR-BI in a manifold manner. First, SR-BI mediates primary attachment of HCV particles of intermediate density to cells. These initial interactions involve apolipoproteins, such as apolipoprotein E, present on the surface of HCV particles, but not the E2 glycoprotein, suggesting that lipoprotein components in the virion act as host-derived ligands for important entry factors such as SR-BI. Second, we found that in contrast to this initial attachment, SR-BI mediates entry of HCV particles independent of their buoyant density. This function of SR-BI does not depend on E2/SR-BI interaction but relies on the lipid transfer activity of SR-BI, probably by facilitating entry steps along with other HCV entry co-factors. Finally, our results underscore a third function of SR-BI governed by specific residues in hypervariable region 1 of E2 leading to enhanced cell entry and depending on SR-BI ability to bind to E2.

Subjects

Subjects :
Apolipoprotein E
Very low-density lipoprotein
MESH: Virus Internalization
Hepacivirus
medicine.disease_cause
Biochemistry
MESH: Protein Structure, Tertiary
Mice
0302 clinical medicine
Viral Envelope Proteins
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Animals
MESH: Hepacivirus
Receptor
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
chemistry.chemical_classification
0303 health sciences
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
virus diseases
Scavenger Receptors, Class B
MESH: Apolipoproteins E
3. Good health
Cell biology
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
lipids (amino acids, peptides, and proteins)
030211 gastroenterology & hepatology
MESH: Cell Line, Tumor
MESH: Rats
Viral protein
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Biology
Microbiology
Apolipoproteins E
03 medical and health sciences
Cell Line, Tumor
medicine
Animals
Humans
Scavenger receptor
MESH: Mice
Molecular Biology
030304 developmental biology
MESH: Humans
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Cell Biology
Virus Internalization
[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
MESH: Scavenger Receptors, Class B
digestive system diseases
Protein Structure, Tertiary
Rats
chemistry
MESH: Viral Envelope Proteins
Glycoprotein
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Lipoprotein

Details

ISSN :
00219258 and 1083351X
Volume :
287
Database :
OpenAIRE
Journal :
Journal of Biological Chemistry
Accession number :
edsair.doi.dedup.....52e1a22ec888ffbb041a49a3abb40db5
Full Text :
https://doi.org/10.1074/jbc.m112.365924