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Minimizing antibody cross-reactivity in multiplex detection of biomarkers in paper-based point-of-care assays
- Source :
- Nanoscale. 8(15)
- Publication Year :
- 2016
-
Abstract
- Highly multiplexed immunoassays could allow convenient screening of hundreds or thousands of protein biomarkers simultaneously in a clinical sample such as serum or plasma, potentially allowing improved diagnostic accuracy and clinical management of many conditions such as autoimmune disorders, infections, and several cancers. Currently, antibody microarray-based tests are limited in part due to cross reactivity from detection antibody reagents. Here we present a strategy that reduces the cross-reactivity between nanoparticle-bound reporter antibodies through the application of ultrasound energy. By this concept, it was possible to achieve a sensitivity 10(3)-fold (5 pg mL(-1)) lower than when no ultrasound was applied (50 ng mL(-1)) for the simultaneous detection of three different antigens. The detection limits and variability achieved with this technique rival those obtained with other types of multiplex sandwich assays.
- Subjects :
- 0301 basic medicine
Antibody microarray
Point-of-Care Systems
Metal Nanoparticles
02 engineering and technology
Biology
Cross Reactions
medicine.disease_cause
Cross-reactivity
Sensitivity and Specificity
03 medical and health sciences
Antigen
Limit of Detection
medicine
Humans
General Materials Science
Multiplex
Ultrasonics
Point of care
Detection limit
Immunoassay
medicine.diagnostic_test
021001 nanoscience & nanotechnology
030104 developmental biology
Immunology
biology.protein
Gold
Antibody
0210 nano-technology
Antibodies, Immobilized
Biomarkers
Subjects
Details
- ISSN :
- 20403372
- Volume :
- 8
- Issue :
- 15
- Database :
- OpenAIRE
- Journal :
- Nanoscale
- Accession number :
- edsair.doi.dedup.....52ceba39ad3d7f5fca8ab60091be5a41