Additional file 6 of Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury

Additional file 6: Supplementary Figure 6. (A) Cerebral edema was measured by brain water content. Quantification analysis showing TBI increased brain edema in the ipsilateral hemisphere on day 3 after TBI, which is significantly alleviated after PS treatment. n = 8 mice per group. (B) Representative images of Nissl staining in the ipsilateral cortex from the sham, TBI + Vehicle (TBI + V), TBI + protein S (TBI + PS) groups, respectively. (C) Quantification analysis showing TBI caused a significant decrease in the number of Nissl-positive cells in the ipsilateral cortex at 3 d post-TBI, and PS application significantly increased the number of Nissl-positive cells in the injured cortex after TBI. n = 6 mice per group. Scale bar = 20 μm. (D) Representative images of Fluoro-Jade B (FJB) staining in the ipsilateral cortex from the sham, TBI + V, TBI + PS groups, respectively. (E) Quantification analysis showing TBI caused a significant increase in the number of FJB-positive cells in the ipsilateral cortex at 3 d post-TBI, and PS application significantly decreased the number of FJB-positive cells in the injured cortex after TBI. n = 6 mice per group. Scale bar = 15 μm. In A, C, E, data are presented as Mean ± SD; **, p < 0.01; ***, p < 0.001. one-way ANOVA followed by Bonferroni’s post-hoc tests.