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Local and regional mechanical characterisation of a collagen-glycosaminoglycan scaffold using high-resolution finite element analysis
- Source :
- Journal of the mechanical behavior of biomedical materials. 3(4)
- Publication Year :
- 2009
-
Abstract
- Artificial tissue growth requires cells to proliferate and differentiate within the host scaffold. As cell function is governed by mechano-sensitive selection, tissue type is influenced by the microscopic forces exposed to the cells, which is a product of macroscopically straining the scaffold. Accordingly, the microscopic strain environment within a CG scaffold is offered here. Using μ CT to characterise CG scaffold architecture, two high-resolution 3D FE models were used to predict the deformation mechanics. While also providing an analysis of region-specific features, such as relative density, pore diameters and microstructural elastic stability, the deformation patterns afforded strains to be inferred for seeded cells. The results indicate a regional dependence, in terms of architectural and mechanical properties. Specifically, the peripheral regions demonstrated the lowest volume fraction, the highest stress concentrations and the greatest potential for elastic instability. Conversely, the mid-region exhibited the most homogeneous environment. Based on the proviso of mechano-sensitive proliferation and differentiation, the findings suggest cell function will vary between CG scaffold regions. Further work should investigate the possibility of improving the fabrication process in order to deliver a construct in line with the mid-region, or alternatively, isolation of the mid-region may prove beneficial for cell culturing.
- Subjects :
- Scaffold
Materials science
Elastic instability
pore-size
Finite Element Analysis
Biomedical Engineering
Biocompatible Materials
tissue-engineering scaffolds
Models, Biological
3-dimensional scaffolds
Biomaterials
Tissue engineering
marrow stromal cells
Physical Stimulation
Materials Testing
Relative density
chondrogenic differentiation
Stress concentration
Glycosaminoglycans
Deformation (mechanics)
Tissue Engineering
Tissue Scaffolds
gag scaffolds
perfusion bioreactor
matrix
Elasticity
Biomechanical Phenomena
Mechanics of Materials
Cell culture
trabecular bone
Volume fraction
Microscopy, Electron, Scanning
Collagen
Shear Strength
Porosity
Biomedical engineering
cross-linking
Subjects
Details
- ISSN :
- 18780180
- Volume :
- 3
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of the mechanical behavior of biomedical materials
- Accession number :
- edsair.doi.dedup.....529ef4ad577020f7b16c822226ba8b21