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Identification of AglE, a Second Glycosyltransferase Involved in N-Glycosylation of the Haloferax volcanii S-Layer Glycoprotein

Authors :
Mehtap Abu-Qarn
Francesca Battaglia
Andrej Trauner
Paul G. Hitchen
Assunta Giordano
Anne Dell
Howard R. Morris
Jerry Eichler
Source :
Journal of bacteriology, 190 (2008): 3140–3146. doi:10.1128/JB.00056-08, info:cnr-pdr/source/autori:Abu-Qarn M; Giordano A; Battaglia F; Trauner A; Hitchen PG; Morris HR; Dell A; Eichler J/titolo:Identification of AglE, a Second Glycosyltransferase Involved in N-Glycosylation of the Haloferax volcanii S-Layer Glycoprotein/doi:10.1128%2FJB.00056-08/rivista:Journal of bacteriology (Print)/anno:2008/pagina_da:3140/pagina_a:3146/intervallo_pagine:3140–3146/volume:190
Publication Year :
2008
Publisher :
American Society for Microbiology [etc.], [Washington, D.C., etc.], Stati Uniti d'America, 2008.

Abstract

Archaea , like Eukarya and Bacteria , are able to N glycosylate select protein targets. However, in contrast to relatively advanced understanding of the eukaryal N glycosylation process and the information being amassed on the bacterial process, little is known of this posttranslational modification in Archaea . Toward remedying this situation, the present report continues ongoing efforts to identify components involved in the N glycosylation of the Haloferax volcanii S-layer glycoprotein. By combining gene deletion together with mass spectrometry, AglE, originally identified as a homologue of murine Dpm1, was shown to play a role in the addition of the 190-Da sugar subunit of the novel pentasaccharide decorating the S-layer glycoprotein. Topological analysis of an AglE-based chimeric reporter assigns AglE as an integral membrane protein, with its N terminus and putative active site facing the cytoplasm. These finding, therefore, contribute to the developing picture of the N glycosylation pathway in Archaea .

Details

Language :
English
Database :
OpenAIRE
Journal :
Journal of bacteriology, 190 (2008): 3140–3146. doi:10.1128/JB.00056-08, info:cnr-pdr/source/autori:Abu-Qarn M; Giordano A; Battaglia F; Trauner A; Hitchen PG; Morris HR; Dell A; Eichler J/titolo:Identification of AglE, a Second Glycosyltransferase Involved in N-Glycosylation of the Haloferax volcanii S-Layer Glycoprotein/doi:10.1128%2FJB.00056-08/rivista:Journal of bacteriology (Print)/anno:2008/pagina_da:3140/pagina_a:3146/intervallo_pagine:3140–3146/volume:190
Accession number :
edsair.doi.dedup.....5280fde7f01556e8205cff26b3935834
Full Text :
https://doi.org/10.1128/JB.00056-08