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Mesenchymal stem cells protective effect in adriamycin model of nephropathy
- Source :
- Cell Transplantation, Vol 17 (2008), ResearcherID
- Publication Year :
- 2009
-
Abstract
- Mesenchymal stem cells (MSCs) may be of value in regeneration of renal tissue after damage; however, lack of biological knowledge and variability of results in animal models limit their utilization. We studied the effects of MSCs on podocytes in vitro and in vivo utilizing adriamycin (ADR) as a model of renal toxicity. The in vivo experimental approach was carried out in male Sprague-Dawley rats (overall 60 animals) treated with different ADR schemes to induce acute and chronic nephrosis. MSCs were given a) concomitantly to ADR in tail vein or b) in aorta and c) in tail vein 60 days after ADR. Homing was assessed with PKH26-MSCs. MSCs rescued podocytes from apoptosis induced by ADR in vitro. The maximal effect (80% rescue) was obtained with MSCs/podocytes coculture ratio of 1:1 for 72 h. All rats treated with ADR developed nephrosis. MSCs did not modify the clinical parameters (i.e., proteinuria, serum creatinine, lipids) but protected the kidney from severe glomerulosclerosis when given concomitantly to ADR. Rats given MSCs 60 days after ADR developed the same severe renal damage. Only a few MSCs were found in renal tubule-interstitial areas 1–24 h after injection and no MSCs were detected in glomeruli. MSCs reduced apoptosis of podocytes treated with ADR in vitro. Early and repeated MSCs infusion blunted glomerular damage in chronic ADR-induced nephropathy. MSCs did not modify proteinuria and progression to renal failure, which implies lack of regenerative potential in this model.
- Subjects :
- Male
Nephrosis
Biomedical Engineering
lcsh:Medicine
Pharmacology
Mesenchymal Stem Cell Transplantation
Nephropathy
Rats, Sprague-Dawley
chemistry.chemical_compound
In vivo
Cell Movement
Medicine
Animals
Humans
Cells, Cultured
Transplantation
Creatinine
Kidney
Antibiotics, Antineoplastic
business.industry
Podocytes
lcsh:R
Mesenchymal stem cell
Glomerulosclerosis
Mesenchymal Stem Cells
Cell Biology
medicine.disease
Rats
Disease Models, Animal
medicine.anatomical_structure
chemistry
Cytoprotection
Doxorubicin
Immunology
Chronic Disease
Kidney Diseases
business
Nephrotic syndrome
Subjects
Details
- ISSN :
- 09636897
- Volume :
- 17
- Issue :
- 10-11
- Database :
- OpenAIRE
- Journal :
- Cell transplantation
- Accession number :
- edsair.doi.dedup.....527a2c075eb969420480fca263ba082f