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Numerical simulation of scaling-up an inverted frusto-conical shaking bioreactor with low shear stress for mammalian cell suspension culture
- Source :
- Cytotechnology. 71:671-678
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Shear stress is one of the key factors affecting the large-scale culture of mammalian cells. In this study, numerical simulation based on computational fluid dynamics was used to conduct a flow-field analysis of 7, 50, 200, and 1200 L inverted frusto-conical shaking bioreactors. The results show that the shear rate, specific mass transfer area (a), and volumetric oxygen mass transfer coefficient (k(L)a) gradually decreased as the scale of the bioreactor increased. Through application of BHK21 and CHO cells in 7, 200, and 1200 L bioreactors, it was found that the cell density and antibody expression level increased as the volume of the bioreactor increased. Moreover, the antibody expression level in a 1200 L bioreactor was nearly 30% and 35% higher than that of 7 and 200 L bioreactors, respectively. The results demonstrate that the environment with a larger volume is more suitable for the growth and antibody expression of CHO cells, indicating shear stress might be the most critical factor affecting the scale-up of mammalian cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10616-019-00308-3) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Computer simulation
Chemistry
Short Communication
Chinese hamster ovary cell
Clinical Biochemistry
technology, industry, and agriculture
Biomedical Engineering
Bioengineering
Cell Biology
equipment and supplies
Shear rate
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Volume (thermodynamics)
030220 oncology & carcinogenesis
SCALE-UP
Shear stress
Bioreactor
Biophysics
Scaling
Biotechnology
Subjects
Details
- ISSN :
- 15730778 and 09209069
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Cytotechnology
- Accession number :
- edsair.doi.dedup.....527682aeaa89f12f9ff17f04c4130476