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New deletion mutant of bacteriophage T5
- Source :
- Journal of Virology. 36:622-626
- Publication Year :
- 1980
- Publisher :
- American Society for Microbiology, 1980.
-
Abstract
- Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DNA. This segment occurs between the early and late gene regions of T5 DNA.
- Subjects :
- Recombination, Genetic
Genes, Viral
Deletion mutant
biology
Immunology
Mutant
biology.organism_classification
Microbiology
Molecular biology
chemistry.chemical_compound
chemistry
Virology
Insect Science
DNA, Viral
Mutation
Agarose gel electrophoresis
T-Phages
Denaturation (biochemistry)
Bacteriophage T5
Late gene
In vitro recombination
DNA
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....526055311aad313b8fe3082cb6bd9336