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A BLUS1 kinase signal and a decrease in intercellular CO(2) concentration are necessary for stomatal opening in response to blue light
- Source :
- Plant Cell
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- Light-induced stomatal opening stimulates CO2 uptake and transpiration in plants. Weak blue light under strong red light effectively induces stomatal opening. Blue light-dependent stomatal opening initiates light perception by phototropins, and the signal is transmitted to a plasma membrane H+-ATPase in guard cells via BLUE LIGHT SIGNALING 1 (BLUS1) kinase. However, it is unclear how BLUS1 transmits the signal to H+-ATPase. Here, we characterized BLUS1 signaling in Arabidopsis thaliana, and showed that the BLUS1 C-terminus acts as an auto-inhibitory domain and that phototropin-mediated Ser-348 phosphorylation within the domain removes auto-inhibition. C-Terminal truncation and phospho-mimic Ser-348 mutation caused H+-ATPase activation in the dark, but did not elicit stomatal opening. Unexpectedly, the plants exhibited stomatal opening under strong red light and stomatal closure under weak blue light. A decrease in intercellular CO2 concentration via red light-driven photosynthesis together with H+-ATPase activation caused stomatal opening. Furthermore, phototropins caused H+-ATPase dephosphorylation in guard cells expressing constitutive signaling variants of BLUS1 in response to blue light, possibly for fine-tuning stomatal opening. Overall, our findings provide mechanistic insights into the blue light regulation of stomatal opening.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Phototropins
Phototropin
Light
Arabidopsis
Plant Science
Photosynthesis
Models, Biological
01 natural sciences
In Brief
Dephosphorylation
Phosphoserine
03 medical and health sciences
Protein Domains
Guard cell
Arabidopsis thaliana
Phosphorylation
Research Articles
Transpiration
biology
Arabidopsis Proteins
Phosphotransferases
Cell Biology
Carbon Dioxide
Plants, Genetically Modified
biology.organism_classification
Proton-Translocating ATPases
030104 developmental biology
Mutation
Plant Stomata
Biophysics
Intracellular
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Plant Cell
- Accession number :
- edsair.doi.dedup.....5248e8aac804837a7636c51e022e5fc0