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Modeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics
- Source :
- Cell Reports, Vol 12, Iss 3, Pp 525-534 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier, 2015.
-
Abstract
- SummaryDespite the increasing use of optogenetics in vivo, the effects of direct light exposure to brain tissue are understudied. Of particular concern is the potential for heat induced by prolonged optical stimulation. We demonstrate that high-intensity light, delivered through an optical fiber, is capable of elevating firing rate locally, even in the absence of opsin expression. Predicting the severity and spatial extent of any temperature increase during optogenetic stimulation is therefore of considerable importance. Here, we describe a realistic model that simulates light and heat propagation during optogenetic experiments. We validated the model by comparing predicted and measured temperature changes in vivo. We further demonstrate the utility of this model by comparing predictions for various wavelengths of light and fiber sizes, as well as testing methods for reducing heat effects on neural targets in vivo.
- Subjects :
- Opsin
Heat induced
Optical fiber
Materials science
Hot Temperature
Light
Brain tissue
Optogenetics
General Biochemistry, Genetics and Molecular Biology
Article
law.invention
03 medical and health sciences
Mice
0302 clinical medicine
Spatio-Temporal Analysis
law
In vivo
Animals
lcsh:QH301-705.5
030304 developmental biology
Neurons
0303 health sciences
Dynamics (mechanics)
Brain
Models, Theoretical
lcsh:Biology (General)
Biophysics
Spatial extent
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 22111247
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....523d3727fc1b9f333218e155bc261aba