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Intra-species cross-reactivity of house dust mite allergens separated by protein blotting and detected by selective elution of mite components and IgE antibodies
- Source :
- Electrophoresis. 14(9)
- Publication Year :
- 1993
-
Abstract
- A complex and varied antibody response to allergens, in particular Dermatophagoides pteronyssinus, is observed in the serum of allergic patients. To examine the role of cross-reacting antigenic determinants in allergy, a strategy has been devised for isolating antibody populations that bind to individual polypeptides of an allergen source separated by sodium dodecyl sulfate-polyacrylamide electrophoresis and electroblotted to nitrocellulose. Polypeptides are identified on the blots, desorbed and coupled to CNBr-activated paper discs. The coupled discs are then used as immunoadsorbent surfaces for the binding of antibodies from allergic sera. Antibodies eluted from the polypeptide immunoadsorbents are then used to reprobe protein blots of the allergen. Results are presented which demonstrate strong cross-reaction between two major mite allergens, Der p I and Der p III, and some weaker intra-species cross-reactivities.
- Subjects :
- Paper
Clinical Biochemistry
Blotting, Western
Immunoglobulin E
medicine.disease_cause
Biochemistry
Cross-reactivity
Analytical Chemistry
Microbiology
Allergen
Antigen
Species Specificity
Antibody Specificity
Mite
medicine
Animals
Humans
Cyanogen Bromide
Immunosorbent Techniques
House dust mite
Mites
biology
Pyroglyphidae
Dust
Allergens
biology.organism_classification
biology.protein
Antibody
Subjects
Details
- ISSN :
- 01730835
- Volume :
- 14
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Electrophoresis
- Accession number :
- edsair.doi.dedup.....523bfbb51b22c225c0107835d1dff0bd