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Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton
- Source :
- PLoS ONE, Vol 16, Iss 3, p e0247593 (2021), PLoS ONE
- Publication Year :
- 2020
-
Abstract
- Segregation distortion (SD) is a genetic mechanism commonly found in segregating or stable populations. The principle behind this puzzles many researchers. The F2generation developed from wildGossypium darwiniiandG.hirsutumCCRI12 species was used to investigate the possible transcription factors within the segregation distortion regions (SDRs). The 384 out of 2763 markers were distorted in 29 SDRs on 18 chromosomes. Good collinearity was observed among genetic and physical maps ofG.hirsutumandG.barbadensesyntenic blocks. Total 568 genes were identified from SDRs of 18 chromosomes. Out of these genes, 128 belonged to three top-ranked salt-tolerant gene families. The DUF597 contained 8 uncharacterized genes linked to Pkinase (PF00069) gene family in the phylogenetic tree, while 15 uncharacterized genes clustered with the zinc finger gene family. Two hundred thirty four miRNAs targeted numerous genes, including ghr-miR156, ghr-miR399 and ghr-miR482, while others targeted top-ranked stress-responsive transcription factors. Moreover, these genes were involved in the regulation of numerous stress-responsive cis-regulatory elements. The RNA sequence data of fifteen upregulated genes were verified through the RT-qPCR. The expression profiles of two highly upregulated genes (Gh_D01G2015andGh_A01G1773) in salt-tolerantG.darwiniishowed antagonistic expression inG.hirsutum. The results indicated that salt-tolerant genes have been possibly transferred from the wildG.darwiniispecies. A detailed functional analysis of these genes can be carried out which might be helpful in the future for gene cloning, transformation, gene editing and the development of salt-resistant cotton varieties.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Cotton
Plant Science
Biochemistry
01 natural sciences
Gene Frequency
Gene Expression Regulation, Plant
Plant Resistance to Abiotic Stress
Chromosome Segregation
Gossypium darwinii
Flowering Plants
Phylogeny
Data Management
Plant Proteins
Genetics
Zinc finger
Multidisciplinary
Ecology
biology
Phylogenetic tree
Eukaryota
Chromosome Mapping
Phylogenetic Analysis
Genomics
Salt Tolerance
Plants
Up-Regulation
Nucleic acids
Phylogenetics
RNA, Plant
Plant Physiology
Medicine
Research Article
Computer and Information Sciences
Science
Research and Analysis Methods
Genes, Plant
Synteny
03 medical and health sciences
Gene mapping
Plant-Environment Interactions
Gene family
Plant Defenses
Evolutionary Systematics
Molecular Biology Techniques
Non-coding RNA
Molecular Biology
Gene
Alleles
Taxonomy
Natural antisense transcripts
Evolutionary Biology
Gossypium
Biology and life sciences
Base Sequence
Plant Ecology
Gene Mapping
Ecology and Environmental Sciences
Organisms
Computational Biology
Plant Pathology
Genome Analysis
biology.organism_classification
Gene regulation
MicroRNAs
030104 developmental biology
RNA
Gene expression
Transcriptome
010606 plant biology & botany
Transcription Factors
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....52384deb20c6a3166071723134eeee15