Back to Search
Start Over
Multispot, label-free biodetection at a phantom plastic-water interface
- Source :
- Proceedings of the National Academy of Sciences of the United States of America 110 (2013): 9350–9355. doi:10.1073/pnas.1214589110, info:cnr-pdr/source/autori:Giavazzi, Fabio; Salina, Matteo; Cerbino, Roberto; Bassi, Mattia; Prosperi, Davide; Ceccarello, Erica; Damin, Francesco; Sola, Laura; Rusnati, Marco; Chiari, Marcella; Chini, Bice; Bellini, Tommaso; Buscaglia, Marco/titolo:Multispot, label-free biodetection at a phantom plastic-water interface/doi:10.1073%2Fpnas.1214589110/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2013/pagina_da:9350/pagina_a:9355/intervallo_pagine:9350–9355/volume:110
- Publication Year :
- 2013
- Publisher :
- The Academy, Washington, D.C. , Stati Uniti d'America, 2013.
-
Abstract
- Recognizing and quantifying specific biomolecules in aqueous samples are constantly needed in research and diagnostic laboratories. As the typical detection procedures are rather lengthy and involve the use of labeled secondary antibodies or other agents to provide a signal, efforts have been made over the last 10 y to develop alternative label-free methods that enable direct detection. We propose and demonstrate an extremely simple, low-cost, label-free biodetector based on measuring the intensity of light reflected by the interface between a fluid sample and an amorphous fluoropolymer substrate having a refractive index very close to that of water and hosting various antibodies immobilized in spots. Under these index-matching conditions, the amount of light reflected by the interface allows straightforward quantification of the amount of antigen binding to each spot. Using antibodies targeting heterologous immunoglobulins and antigens commonly used as markers for diagnoses of hepatitis B and HIV, we demonstrate the limit of detection of a few picograms per square millimeter of surface-bound molecules. We also show that direct and real-time access to the amount of binding molecules allows the precise extrapolation of adhesion rates, from which the concentrations of antigens in solution can be estimated down to fractions of nanograms per milliliter.
- Subjects :
- Materials science
Light
Optical Phenomena
Interface (computing)
Protein Array Analysis
Human immunodeficiency virus (HIV)
HIV Infections
Nanotechnology
medicine.disease_cause
label-free
Antibodies
Chemistry Techniques, Analytical
Imaging phantom
medicine
Humans
reflective phantom interface
immunoassay
Antigens
Label free
chemistry.chemical_classification
Detection limit
Multidisciplinary
biology
optical biosensor
Biomolecule
Water
Biological Sciences
Hepatitis B
Antigen binding
Primary and secondary antibodies
BIO/10 - BIOCHIMICA
diagnostica biomedica
chemistry
protein microarray
biology.protein
Biosensori
biomolecular detection
Plastics
anticorpi
Biomarkers
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America 110 (2013): 9350–9355. doi:10.1073/pnas.1214589110, info:cnr-pdr/source/autori:Giavazzi, Fabio; Salina, Matteo; Cerbino, Roberto; Bassi, Mattia; Prosperi, Davide; Ceccarello, Erica; Damin, Francesco; Sola, Laura; Rusnati, Marco; Chiari, Marcella; Chini, Bice; Bellini, Tommaso; Buscaglia, Marco/titolo:Multispot, label-free biodetection at a phantom plastic-water interface/doi:10.1073%2Fpnas.1214589110/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2013/pagina_da:9350/pagina_a:9355/intervallo_pagine:9350–9355/volume:110
- Accession number :
- edsair.doi.dedup.....51fdf20bfbbc72b6c209f598e8e4f11d