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Abnormal lipid/lipoprotein metabolism and high plasma testosterone levels in male but not female aromatase-knockout mice
- Source :
- Archives of biochemistry and biophysics. 622
- Publication Year :
- 2016
-
Abstract
- Sex steroid hormones, such as estrogen and testosterone, are believed to play important roles in lipid metabolism. To elucidate the effects of estrogen depletion on lipid metabolism in male and female mice, we used aromatase-knockout (ArKO) mice, in which Cyp19 gene disruption prevented estrogen synthesis in vivo. These mice were divided into the following 4 groups: male and female ArKO mice and male and female wild-type (WT) mice. These mice were fed a normal-fat diet (13.6% fat) ad libitum. At 159 days after birth, the mice were tested for liver and plasma lipid content and hepatic hormone receptor- and lipid/lipoprotein metabolism-related gene expression. Interestingly, we found that hepatic steatosis was accompanied by markedly elevated plasma testosterone levels in male ArKO mice but not in female ArKO mice. Plasma lipoprotein profiles exhibited concurrent decreases in LDL- and small dense LDL-triglyceride (TG) levels in male ArKO mice. Moreover, male mice, but not female mice, exhibited marked elevations in androgen receptor (AR), sterol regulatory element-binding protein 1 (SREBP1), and CD36 expression. These results strongly suggest that Cyp19 gene disruption, which induces a sexually dimorphic response and high plasma testosterone levels in male mice, also induces hepatic steatosis.
- Subjects :
- 0301 basic medicine
CD36 Antigens
Male
medicine.medical_specialty
medicine.drug_class
CD36
Lipoproteins
Biophysics
030209 endocrinology & metabolism
Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Aromatase
Internal medicine
medicine
Animals
Testosterone
Molecular Biology
Mice, Knockout
biology
Lipid metabolism
Estrogens
medicine.disease
Lipid Metabolism
Up-Regulation
Androgen receptor
Fatty Liver
030104 developmental biology
Endocrinology
Liver
Estrogen
Hormone receptor
Receptors, Androgen
biology.protein
Female
Steatosis
Sterol Regulatory Element Binding Protein 1
Subjects
Details
- ISSN :
- 10960384
- Volume :
- 622
- Database :
- OpenAIRE
- Journal :
- Archives of biochemistry and biophysics
- Accession number :
- edsair.doi.dedup.....51fd38a7be0ee26cb0da72427d5cc097