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Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
- Source :
- Journal of Visualized Experiments, ISSUE=123;TITLE=Journal of Visualized Experiments, Journal of visualized experiments, 2017(123):e55587. MYJoVE Corporation, Journal of Visualized Experiments : JoVE
- Publication Year :
- 2017
- Publisher :
- MyJove Corporation, 2017.
-
Abstract
- Cardiomyocytes can now be derived with high efficiency from both human embryonic and human induced-Pluripotent Stem Cells (hPSC). hPSC-derived cardiomyocytes (hPSC-CMs) are increasingly recognized as having great value for modeling cardiovascular diseases in humans, especially arrhythmia syndromes. They have also demonstrated relevance as in vitro systems for predicting drug responses, which makes them potentially useful for drug-screening and discovery, safety pharmacology and perhaps eventually for personalized medicine. This would be facilitated by deriving hPSC-CMs from patients or susceptible individuals as hiPSCs. For all applications, however, precise measurement and analysis of hPSC-CM electrical properties are essential for identifying changes due to cardiac ion channel mutations and/or drugs that target ion channels and can cause sudden cardiac death. Compared with manual patch-clamp, multi-electrode array (MEA) devices offer the advantage of allowing medium- to high-throughput recordings. This protocol describes how to dissociate 2D cell cultures of hPSC-CMs to small aggregates and single cells and plate them on MEAs to record their spontaneous electrical activity as field potential. Methods for analyzing the recorded data to extract specific parameters, such as the QT and the RR intervals, are also described here. Changes in these parameters would be expected in hPSC-CMs carrying mutations responsible for cardiac arrhythmias and following addition of specific drugs, allowing detection of those that carry a cardiotoxic risk.
- Subjects :
- 0301 basic medicine
General Chemical Engineering
Cell Culture Techniques
Pharmacology
safety pharmacology
Medicine
Myocyte
Myocytes, Cardiac
Micro-electrode array
Induced pluripotent stem cell
Cells, Cultured
Cultured
Safety pharmacology
General Neuroscience
3. Good health
field potential
Issue 123
RR interval
Electrophysiologic Techniques
Stem cell
Electrophysiologic Techniques, Cardiac
Cardiac
Pluripotent Stem Cells
QT interval
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)
Long QT syndrome
Cells
Multi-electrode array
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
cardiac arrhythmia
long QT syndrome
Humans
drug screening
Electrodes
Myocytes
General Immunology and Microbiology
business.industry
multi-electrode arrays
Cardiac arrhythmia
medicine.disease
Developmental biology
Drug screening
Electrophysiology
Field potential
Micro-electrode arrays
Multi-electrode arrays
electrophysiology
Embryonic stem cell
030104 developmental biology
micro-electrode arrays
business
Neuroscience
Developmental Biology
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....51fac33e9d68c17e4b583d91dd576bb1