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Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding
- Source :
- BMC Plant Biology, BMC Plant Biology, Vol 19, Iss 1, Pp 1-15 (2019)
- Publication Year :
- 2019
- Publisher :
- Research Square Platform LLC, 2019.
-
Abstract
- Background In water lily (Nymphaea) hybrid breeding, breeders often encounter non-viable seeds, which make it difficult to transfer desired or targeted genes of different Nymphaea germplasm. We found that pre-fertilization barriers were the main factor in the failure of the hybridization of Nymphaea. The mechanism of low compatibility between the pollen and stigma remains unclear; therefore, we studied the differences of stigma transcripts and proteomes at 0, 2, and 6 h after pollination (HAP). Moreover, some regulatory genes and functional proteins that may cause low pollen-pistil compatibility in Nymphaea were identified. Results RNA-seq was performed for three comparisons (2 vs 0 HAP, 6 vs 2 HAP, 6 vs 0 HAP), and the number of differentially expressed genes (DEGs) was 8789 (4680 were up-regulated), 6401 (3020 were up-regulated), and 11,284 (6148 were up-regulated), respectively. Using label-free analysis, 75 (2 vs 0 HAP) proteins (43 increased and 32 decreased), nine (6 vs 2 HAP) proteins (three increased and six decreased), and 90 (6 vs 0 HAP) proteins (52 increased and 38 decreased) were defined as differentially expressed proteins (DEPs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the DEGs and DEPs were mainly involved in cell wall organization or biogenesis, S-adenosylmethionine (SAM) metabolism, hydrogen peroxide decomposition and metabolism, reactive oxygen species (ROS) metabolism, secondary metabolism, secondary metabolite biosynthesis, and phenylpropanoid biosynthesis. Conclusions Our transcriptomic and proteomic analysis highlighted specific genes, incuding those in ROS metabolism, biosynthesis of flavonoids, SAM metabolism, cell wall organization or biogenesis and phenylpropanoid biosynthesis that warrant further study in investigations of the pollen-stigma interaction of water lily. This study strengthens our understanding of the mechanism of low pollen-pistil compatibility in Nymphaea at the molecular level, and provides a theoretical basis for overcoming the pre-fertilization barriers in Nymphaea in the future.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Interspecific reproductive barriers
Proteome
Pollen-stigma interaction
Plant Science
Flowers
01 natural sciences
Transcriptome
03 medical and health sciences
lcsh:Botany
Nymphaea
KEGG
Secondary metabolism
Regulator gene
Plant Proteins
Flavonoids
Phenylpropanoid
biology
Proteomic
food and beverages
ROS
biology.organism_classification
lcsh:QK1-989
Plant Breeding
030104 developmental biology
Gene Ontology
Cell wall organization
Biochemistry
Transcriptomic
Hybridization, Genetic
Pollen
010606 plant biology & botany
Research Article
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology, BMC Plant Biology, Vol 19, Iss 1, Pp 1-15 (2019)
- Accession number :
- edsair.doi.dedup.....51f3432970e12c65c4ceaa82e0646a16