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Determinants of kinin release in isolated rat hindquarters
- Source :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 274:R120-R125
- Publication Year :
- 1998
- Publisher :
- American Physiological Society, 1998.
-
Abstract
- We studied the determinants of kinin release into the venous effluent of rat hindquarters perfused with Krebs bicarbonate buffer. Kinin release in preparations perfused with control media (14.6 +/- 2.5-20.7 +/- 6.7 pg/15 min) was surpassed by that in preparations perfused with media containing kininase inhibitors (243 +/- 53 to 276 +/- 78 pg/15 min). Kinin release increased when purified kininogen (from 242 +/- 43 to 3,365 +/- 725 pg/15 min) or kallikrein (from 270 +/- 49 to 30,649 +/- 8,040 pg/15 min) was added to the perfusate. Conversely, kinin release fell when the kallikrein inhibitor aprotinin (from 272 +/- 58 to 122 +/- 27 pg/15 min) or soybean trypsin inhibitor (from 273 +/- 52 to 195 +/- 25 pg/15 min) was added. Both basal and kininogen-induced kinin release were attenuated in preparations perfused with media containing cycloheximide, a protein synthesis inhibitor, but kallikrein-induced kinin release was not. These data suggest that kinin release from perfused rat hindquarters reflects the activity of both the kinin-degrading and kinin-generating pathways and that the latter is sustained by a kallikrein manufactured de novo and by preexistent kininogen(s).
- Subjects :
- Male
medicine.medical_specialty
Time Factors
Physiology
Bradykinin
Kinins
In Vitro Techniques
Cycloheximide
Biology
Rats, Sprague-Dawley
chemistry.chemical_compound
Aprotinin
Physiology (medical)
Internal medicine
medicine
Animals
cardiovascular diseases
Kininogen
Kunitz STI protease inhibitor
Kininogens
urogenital system
Kallikrein
Kinin
biological factors
Hindlimb
Rats
Perfusion
Endocrinology
chemistry
cardiovascular system
Liberation
Kallikreins
Trypsin Inhibitors
circulatory and respiratory physiology
medicine.drug
Subjects
Details
- ISSN :
- 15221490 and 03636119
- Volume :
- 274
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Accession number :
- edsair.doi.dedup.....51e514c9faa09e9f2e90b7893c57b534