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Localization of nonerythroid spectrin and actin in mouse oocytes and preimplantation embryos
- Source :
- Differentiation; research in biological diversity. 30(1)
- Publication Year :
- 1985
-
Abstract
- Mouse oocytes, cleavage-stage embryos, and blastocyst-stage embryos were studied to show the distribution of both an immunoanalog to nonerythroid spectrin (p 230) and F-actin. Using antibodies to nonerythroid spectrin, diffuse, positive cytoplasmic fluorescence was regularly seen in oocytes and embryo cells. The presence of nonerythroid spectrin in oocytes was confirmed by immunoblotting. Oocytes usually exhibited an inconspicuous submembraneous layer of nonerythroid spectrin, which was more pronounced in the area of the polar body. Oocytes regularly exhibited a peripheral concentration of actin. Throughout the cleavage and blastocyst stages, a cortical layer of nonerythroid spectrin and actin was usually observed in embryo cells. These submembraneous layers on the outer surface of the embryo were relatively thin as compared to those in areas of intercellular contact. The contact areas regularly showed distinct positive staining, including a concentration of label at the most peripheral region of each contact area. This resulted in the presence of ring-like fluorescence around each blastomere. Nonerythroid spectrin and actin showed concentration to the contact area between the oocyte and the polar body. Although the general localization patterns of nonerythroid spectrin and actin were similar, double staining experiments revealed that slightly different planes of focus were necessary to obtain sharp definition of the fluorescence of these components in areas of intercellular contact: the ring-like concentration of nonerythroid spectrin appeared to be localized more peripherally than that of actin. The cells of preimplantation embryos show motile features that include actual cell movements and striking changes in cell shape (e.g., during compaction). The sub-membraneous layers of nonerythroid spectrin and actin may contribute to the regulation of the deformability and thus the shape of embryo cells. Cytoskeletal components are probably involved in the formation of cell contacts and regulation of contact areas between blastomeres, as well as the control of cell movement. Thus, nonerythroid spectrin and actin may play important roles in the regulation of cleavage-stage development and blastocyst transformation.
- Subjects :
- Paper
Cancer Research
Cleavage Stage, Ovum
Embryonic Development
macromolecular substances
Biology
Cleavage (embryo)
03 medical and health sciences
Polar body
Mice
0302 clinical medicine
Pregnancy
medicine
Animals
Spectrin
Blastocyst
Cytoskeleton
Molecular Biology
Actin
030304 developmental biology
0303 health sciences
Collodion
Embryo
Cell Biology
Embryo, Mammalian
Molecular biology
Actins
Cell biology
Molecular Weight
medicine.anatomical_structure
Cytoplasm
Oocytes
Electrophoresis, Polyacrylamide Gel
Female
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 03014681
- Volume :
- 30
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Differentiation; research in biological diversity
- Accession number :
- edsair.doi.dedup.....51e31f8d77ed2b1c02ff28f8258d44b9