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Suppressor of cytokine signalling 6 is a potential regulator of antimicrobial peptides in the Chinese oak silkworm, Antheraea pernyi
- Source :
- Molecular Immunology. 140:12-21
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The SOCS/CIS is a family of intracellular proteins distributed widely among living organisms. The members of this family have extensively been studied in mammals and have been shown to regulate various physiological processes. In contrast, the functional roles of SOCS/CIS family proteins are unknown in most invertebrates, including insects. Here, we retrieved a full-length open reading frame (ORF) of SOCS-6 from Chines oak silkworm, Antheraea pernyi (Designated as ApSOCS-6), using the RNA-seq database. The predicted ApSOCS-6 amino acid sequence comprised an N-terminal SH2 domain and a C-terminal SOCS-box domain. It shared the highly conserved structures of the SOCS proteins with other lepidopteran species. ApSOCS-6 mRNA transcript was detected in all the tested tissues of the A. pernyi larvae; however, the highest mRNA levels were found in the larval hemocytes, fat bodies, and integuments. The mRNA transcript levels of ApSOCS-6 were increased in the A. pernyi larval hemocytes and fat bodies after a challenge by the Gram-positive bacteria, M. luteus, Gram-negative bacteria, Escherichia coli, Virus, ApNPV, and Fungus, B. bassiana. After the knockdown of ApSOCS-6, we found a significant increase in bacterial clearance and a decrease in the relative replication of bacteria. To evaluate the possible cause of enhanced antibacterial activity, we measured antimicrobial peptides expression in the fat body of A. pernyi larvae. The production of AMPs was strongly increased in the B. cereus infected larval fat bodies following silencing of ApSOCS-6. Our data indicate that ApSOCS-6 negatively regulates the expression of AMPs in immune tissues in response to the B. cereus challenge.
- Subjects :
- Hemocytes
Time Factors
Avena
Immunology
Antimicrobial peptides
Suppressor of Cytokine Signaling Proteins
Antheraea pernyi
Biology
Suppressor of cytokine signalling
Microbiology
Animals
Tissue Distribution
Amino Acid Sequence
Molecular Biology
Peptide sequence
Phylogeny
Messenger RNA
Gene knockdown
Bacteria
Base Sequence
Gene Expression Profiling
fungi
Fungi
Bombyx
biology.organism_classification
Protein Structure, Tertiary
Open reading frame
Gene Expression Regulation
Insect Proteins
Antimicrobial Peptides
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....51ddda1f0375167a4e34092944e019bd