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Modulation of the antioxidant activity of HO* scavengers by albumin binding: a 19F-NMR study
Modulation of the antioxidant activity of HO* scavengers by albumin binding: a 19F-NMR study
- Source :
- Biochemical and biophysical research communications. 307(4)
- Publication Year :
- 2003
-
Abstract
- The interaction between different HO(z.rad;) radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF(3)PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF(3)PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF(3)PAF from HO* radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF(3)PAF and trolox were found to bind to BSA with association constants in the order of 5 x 10(3)M(-1) and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF(3)PAF.
- Subjects :
- Fluorine Radioisotopes
Antioxidant
medicine.medical_treatment
Radical
Fluoroacetates
Biophysics
Serum albumin
Carnosine
Aminophenols
Biochemistry
Binding, Competitive
Antioxidants
chemistry.chemical_compound
Acetamides
medicine
Organic chemistry
Trifluoroacetic Acid
Bovine serum albumin
Chromans
Molecular Biology
Nuclear Magnetic Resonance, Biomolecular
biology
Chemistry
Hydroxyl Radical
Serum Albumin, Bovine
Cell Biology
Free Radical Scavengers
TFAM
biology.protein
Hydroxyl radical
Trolox
Nuclear chemistry
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 307
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....51db50757d8bc83ebe0c485eb7fcf157