Back to Search Start Over

Modulation of the antioxidant activity of HO* scavengers by albumin binding: a 19F-NMR study

Modulation of the antioxidant activity of HO* scavengers by albumin binding: a 19F-NMR study

Authors :
Silvio Aime
Simona Baroni
Mauro Fasano
Giuseppe Digilio
Valentina Mainero
Erik Bruno
Source :
Biochemical and biophysical research communications. 307(4)
Publication Year :
2003

Abstract

The interaction between different HO(z.rad;) radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF(3)PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF(3)PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF(3)PAF from HO* radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF(3)PAF and trolox were found to bind to BSA with association constants in the order of 5 x 10(3)M(-1) and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF(3)PAF.

Details

ISSN :
0006291X
Volume :
307
Issue :
4
Database :
OpenAIRE
Journal :
Biochemical and biophysical research communications
Accession number :
edsair.doi.dedup.....51db50757d8bc83ebe0c485eb7fcf157