A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

Authors :: Rose G. F. Leke
Ali Salanti
Diane Wallace Taylor
Elise P. Ngati
Yukie M. Lloyd
Source :: Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-7 (2017), Lloyd, Y M, Ngati, E P, Salanti, A, Leke, R G F & Taylor, D W 2017, ' A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum ', Scientific Reports, vol. 7, 14705 . https://doi.org/10.1038/s41598-017-13900-4
Publication Year :: 2017
Publisher :: Nature Publishing Group UK, 2017.
Abstract: Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

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