Back to Search
Start Over
Hippocampal neuronal maturation triggers post-synaptic clustering of brain temperature-sensor TRPV4
- Source :
- Biochemical and Biophysical Research Communications. 458:168-173
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Compartmentalization of neuronal function is achieved via specifically localized clustering of ion channels in discrete subcellular membrane domains. Transient receptor potential (TRP) channels exhibit highly variable cellular and subcellular patterns of expression. We previously revealed that the thermo-sensor TRPV4 (activated above 34 °C) is gated by physiological brain temperatures in hippocampal neurons and thereby controls their excitability. Here, we examined synaptic clustering of TRPV4 in developing hippocampal neurons. We found that TRPV4 accumulated in the soma of immature hippocampal neurons, and did not localize to post-synaptic locations although PSD-95-labeled post-synaptic structures were evident. During the maturation of neurons, TRPV4 was targeted to dendrites and also clustered at post-synaptic locations. Taken together, we reveal that TRPV4 localizes to post-synaptic sites and the post-synaptic targeting is strictly regulated in a neuronal maturation-dependent manner.
- Subjects :
- TRPV4
Biophysics
TRPV Cation Channels
Hippocampus
Hippocampal formation
Neurotransmission
Biology
Synaptic Transmission
Biochemistry
Synapse
Transient receptor potential channel
medicine
Animals
Molecular Biology
Mice, Knockout
Neurons
Gene Expression Regulation, Developmental
Dendrites
Cell Biology
Compartmentalization (psychology)
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
nervous system
Astrocytes
Soma
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 458
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....5144569f3c9085952ac25851d6bddc0b